TOWARDS THE PRODUCTION OF STRESS TOLERANT GRAPEVINE CULTIVARS

The biotic and abiotic stresses are among the major limiting factors in plant productivity.
To overcome these difficulties molecular breeding methods have recently been widely applied to improve the stress- and disease resistance of grapevine cultivars.
To this end we tested the suitability of a ferritin gene derived from Medicago sativa (MsFerr). We propose that by sequestering the intracellular iron involved in generation of the very reactive hydroxyl radicals through Fenton-reaction, the increased overall ferritin concentration results in increased protection of plant cells from oxidative damage induced by a wide range of stresses.
We have improved the existing regeneration and transformation protocols.
In our experiments we raised the number of regenerated plants due to the application of 1 μM benzyladenine (BA). The plant regeneration was supported by the maintenance of plant material on “half MS” medium without selection agent (after two-year selection), and by cut of abnormal embryos under the hypocotyl.
The independent regenerated transformants were tested by PCR, qPCR, Western blot, and were used for various abiotic stress tolerance experiments.

I International Symposium on Biotechnology of Fruit Species: BIOTECHFRUIT2008

A. Zok, R. Oláh, B.P. Kós, E. Hideg, V.G. Horváth, GY. Váradi, E. Szegedi, B. Báló

somatic embryos, genetic transformation, regeneration, oxidative damages, ferritin

https://doi.org/10.17660/ActaHortic.2009.839.89