Articles
FUNCTIONAL DIVERSITY FOR RESPONSE TO WATER DEFICIT AMONG DIFFERENT STRAWBERRY GENOTYPES COMPARED TO THEIR GENETIC STRUCTURE AS ASSESSED BY AFLP MARKERS
Article number
839_67
Pages
491 – 497
Language
English
Abstract
Strawberry (Fragaria × ananassa Duch.) belongs to the rose family (Rosaceae, subfam.
Rosoideae) and is mainly cultivated in the northern hemisphere.
Drought stress is one of the most important environmental factors that limit plant growth and development, thus reducing yield.
The objective of present research was to draw a correlation between the genetic structure of different strawberry species and cultivars as assessed by AFLP markers and plant responses to drought stress.
Experiments were performed in a growth chamber under constant temperature (17°C), RH (60%), and light intensity (around 100-105 µmol m-2 s-1). Leaf water losing rate (WLR, calculated as the leaf weight loss over time), and relative water content (RWC) were compared for 21 cultivars/species, one diploid species F. vesca and one octaploid species F. chiloensis. WLR could discriminate genotypes showing a contrasting response to water stress.
This response allowed clustering strawberry genotypes in susceptible and drought-tolerant groups.
Secondly, the genetic diversity within and among these groups was determined using amplified fragment length polymorphism (AFLP) to evaluate the genetic basis for the observed variation in WLR and RWC. Clustering results based on AFLP markers linked to the RWC and WLR traits indicated an eco-physiological relevant grouping was obtained.
In particular, common correlated AFLP alleles indicate the involvement of common loci of genetic control of these two characters at DNA level.
Rosoideae) and is mainly cultivated in the northern hemisphere.
Drought stress is one of the most important environmental factors that limit plant growth and development, thus reducing yield.
The objective of present research was to draw a correlation between the genetic structure of different strawberry species and cultivars as assessed by AFLP markers and plant responses to drought stress.
Experiments were performed in a growth chamber under constant temperature (17°C), RH (60%), and light intensity (around 100-105 µmol m-2 s-1). Leaf water losing rate (WLR, calculated as the leaf weight loss over time), and relative water content (RWC) were compared for 21 cultivars/species, one diploid species F. vesca and one octaploid species F. chiloensis. WLR could discriminate genotypes showing a contrasting response to water stress.
This response allowed clustering strawberry genotypes in susceptible and drought-tolerant groups.
Secondly, the genetic diversity within and among these groups was determined using amplified fragment length polymorphism (AFLP) to evaluate the genetic basis for the observed variation in WLR and RWC. Clustering results based on AFLP markers linked to the RWC and WLR traits indicated an eco-physiological relevant grouping was obtained.
In particular, common correlated AFLP alleles indicate the involvement of common loci of genetic control of these two characters at DNA level.
Authors
F. Razavi, M.C. van Labeke, J. de Riek
Keywords
Fragaria × ananassa, Fragaria chiloensis, Fragaria vesca, strawberry cultivar, drought stress, water deficit, AFLP markers, genetic relationship, clustering analysis
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