Articles
ROLE OF SAMS GENE IN FE UPTAKE MECHANISM OF MALUS XIAOJINENSIS
Article number
839_63
Pages
463 – 469
Language
English
Abstract
Iron (Fe) is one of the essential elements for plants, including apple tree.
Iron deficiency chlorosis causes, particularly on calcareous soils, a serious impact on growth and production of apple fruits.
It has been proven that the use of Fe-efficient rootstock is a fundamental way to alleviate the disorder.
For this reason, we had screened out in genus Malus and used the Fe-efficient genotype of Malus xiaojinensis. We report here the results about the changes of protein expression of the genotype under Fe deficiency stress and discussed the possible role of MxSAMS gene in Fe uptake of apple.
More than 700 protein spots with high repetition were obtained by 2-DE (two-dimensional electrophoresis) method.
We show that 13 up-regulated protein spots being classed into 11 kinds of proteins were identified by use of MALDI TOF (matrix-assisted laser desorption/ionization-time of flight), PMF (peptide mass fingerprinting) analysis and database.
Combined with results of Fe deficiency subtracted library of M. xiaojinensis, total length of both cDNA and DNA of MxSAMS was cloned by RACE, a SAMS (S-adenolyl-L-methionine synthetase enzyme) gene from the Fe-efficient M. xiaojinensis. The results by RT-PCR showed that expression of MxSAMS in roots or leaves of M. xiaojinensis was induced by Fe deficiency stress.
Iron deficiency chlorosis causes, particularly on calcareous soils, a serious impact on growth and production of apple fruits.
It has been proven that the use of Fe-efficient rootstock is a fundamental way to alleviate the disorder.
For this reason, we had screened out in genus Malus and used the Fe-efficient genotype of Malus xiaojinensis. We report here the results about the changes of protein expression of the genotype under Fe deficiency stress and discussed the possible role of MxSAMS gene in Fe uptake of apple.
More than 700 protein spots with high repetition were obtained by 2-DE (two-dimensional electrophoresis) method.
We show that 13 up-regulated protein spots being classed into 11 kinds of proteins were identified by use of MALDI TOF (matrix-assisted laser desorption/ionization-time of flight), PMF (peptide mass fingerprinting) analysis and database.
Combined with results of Fe deficiency subtracted library of M. xiaojinensis, total length of both cDNA and DNA of MxSAMS was cloned by RACE, a SAMS (S-adenolyl-L-methionine synthetase enzyme) gene from the Fe-efficient M. xiaojinensis. The results by RT-PCR showed that expression of MxSAMS in roots or leaves of M. xiaojinensis was induced by Fe deficiency stress.
Authors
Y. Zhu, Y. Wang, J. Kong, J. Wang, X. Zhang, H. Han
Keywords
apple rootstock, S-adenosylmethionine synthetase, gene function, iron absorption mechanism, iron transportation
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