MICROPROPAGATION, ROOTING AND ACCLIMATIZATION OF BLACKBERRY ‘AGAVAM’

Plant micropropagation of blackberry (Rubus spp.) ‘Agavam’ was carried out in two periods.
In 2008 the cultivars ‘Thornfree’ and ‘Darrow’ also were included in the investigation.
Meristem tip explants from the axillary buds were cut into three sizes: 1 mm.
Two media compositions were used for the establishment of culture in 2006 and six in 2008: Murashige and Skoog mineral salts and vitamins medium (also modified) supplemented with different proportions of 6-BAP, IAA, IBA and GA₃. Also five types of multiplication media were used.
A higher percentage of alive explants was obtained using MS mineral salt and vitamins medium supplemented with BAP 1 mg/L. Establishing a medium consisting of MS salts with ¼ of nitrates, 2x strength Fe salts supplemented with KH₂PO₄ 170 mg/L, thiamine 0.4 mg/L, adenine sulfate 80 mg/L, 6-BAP 2 mg/L, IBA 0.05 mg/L and GA₃ 0.21 mg/L was found as the most universal for the three tested genotypes.
The best multiplication medium was found to be of MS salts where Fe ions were added in the form of Fe EDTA and hormones BAP 1 mg/L and IBA 0.05 mg/L. Rooting was performed using two methods: in vitro and ex vitro in peat substrate.
Rooting performed in peat substrate gave significantly higher outcome of rooted plants than in vitro rooting.

I International Symposium on Biotechnology of Fruit Species: BIOTECHFRUIT2008

L. Lepse, V. Laugale

Rubus spp., in vitro, meristem, media composition, tissue culture

https://doi.org/10.17660/ActaHortic.2009.839.2