Articles

APPLE RIPENING DETERMINED BY CONVENTIONAL METHODS AND THE ‘NSURE’ GENETIC ASSAY

Article number
934_116
Pages
871 – 875
Language
English
Abstract
Molecular biology techniques are developing rapidly and can now be used as assays to help determine the optimum harvest date for fruit crops.
While molecular assays are still more expensive and time consuming than the conventional maturity tests currently used for apples they offer a number of potential advantages, in particular the possibility to detect changes in fruit ripening processes at a very early stage and perhaps in the near future to help predict storage quality outcomes.
To adapt a new methodology for practical use requires trialling and comparison with existing methods.
When results from different seasons and apple cultivars are available, the suitability and reliability of molecular methodology can be better understood and assessed.
This work compares conventional methods for optimum harvest date determination with the genetic testing service, supplied by the Dutch based Company ‘Nsure’. The ‘Nsure’ test measures the expression of genes with relevance to some of the key ripening processes in maturing apples.
In 2008 and 2009, three apple cultivars: ‘Gala’, ‘Fuji’ and ‘Braeburn’ from the Lake Constance growing region of Germany were sampled three times in the period leading up to harvest and two times from unpicked trees after the expected optimum harvest date.
The conven¬tional ripening parameters: fruit firmness, total soluble solids and starch degradation pattern were used to calculate a ‘Streif’ ripening index and the optimum harvest date for long term storage.
In addition, samples for the respiration rate, ethylene production and internal ethylene were taken.
Gene expression at each sampling date was evaluated by ‘Nsure’ Company.
The results from both methods were compared.

Publication
Authors
D.A. Neuwald , J. Streif
Keywords
ethylene, fruit firmness, Malus domestica, optimal harvest date, respiration, starch pattern index, total soluble solids
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