Articles

EXPLORING THE INVOLVEMENT OF ETHYLENE IN THE REGULATION OF COLOR CHANGES IN CITRUS FRUIT

Article number
934_117
Pages
879 – 885
Language
English
Abstract
Citrus fruits produce minute amounts of ethylene during maturation and therefore have been long recognized as non-climacteric.
Evidences indicate that ethylene is playing a role in the control of fruit maturation and, for instance, applica-tion of the gas is a common postharvest practice to accelerate fruit coloration, especially in early-harvested cultivars.
How ethylene is perceived and transduced during maturation of this non-climacteric fruit, and whether the hormone may be an inducer or its perception is required throughout the maturation process have not been yet elucidated.
To address these questions, the ethylene action inhibitor 1-MCP was applied to mandarin fruit (Citrus clementine ‘Clemenules’) both on the tree and also after harvested, and changes in fruit color, carotenoid content and composition, and expression of key genes of carotenoid biosynthesis were analyzed.
Mandarin fruits were treated at three ripening stages: before initiation, at the onset and at the middle of peel degreening.
Application of 1-MCP to fruit on the tree was very effective inhibiting ethylene action, as evidenced by the reduced postharvest response to ethylene 24 h after treatment.
Nevertheless, inhibition of ethylene action in fruit on and off the tree was not always accompanied by a delay in the rate of fruit colouration and by substantial changes in carotenoid content and composition.
However, expression of key carotenoid biosynthetic genes was much more sensitive to inhibition of ethylene action.
Collectively, these results indicate that ethylene action during fruit coloration is mainly exerted through regulation of the expression of carotenoid biosynthetic genes and other downstream metabolic processes appear to be ethylene-independent.

Publication
Authors
L. Carmona, M.J. Rodrigo , L. Zacarías
Keywords
ethylene, 1-MCP, citrus fruit, carotenoids biosynthesis, color changes
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