Articles
GENETIC DIVERSITY OF GREEK WILD AND CULTIVATED POMEGRANATE (PUNICA GRANATUM L.) GENOTYPES AND CULTIVARS USING MOLECULAR MARKERS
Article number
940_25
Pages
193 – 200
Language
English
Abstract
In this study, different pomegranate genotypes and cultivars were discrim-inated and identified using the Random Amplified Polymorphic DNA (RAPD) and the Inter Simple Sequence Repeats (ISSR) molecular markers. 38 different pome-granate genotypes and 2 cultivars were discriminated and identified using RAPD and ISSR molecular markers.
Genomic DNA was extracted from young leaves using the CTAB method. 35 decamer RAPD primers and 15 ISSR primers were tested from which 10 RAPD and 10 ISSR primers, which gave the most polymorphic bands, were used.
Amplified products of PCR were separated by electrophoresis in 3% (w/v) and 5% (w/v) agarose gel equivalent.
NTSYS software, version 2.02, was used to estimate genetic similarities using Jaccards algorithm while the dendrogram was constructed by UPGMA and Neighbor Joining methods.
High genetic similarity between the samples collected from the agricultural area of Pella, and the samples collected from the Institute of Pomology of Naoussa (>0.70), suggests that they might be clones of some cultivars.
The Greek cultivar Ermioni showed high genetic similarity (95%) with cultivar Wonderful (95%). RAPD and ISSR molecular markers were useful for studying the genetic relationships between pomegranate genotypes and cultivars, with the ISSR markers generating more polymorphic bands.
Genomic DNA was extracted from young leaves using the CTAB method. 35 decamer RAPD primers and 15 ISSR primers were tested from which 10 RAPD and 10 ISSR primers, which gave the most polymorphic bands, were used.
Amplified products of PCR were separated by electrophoresis in 3% (w/v) and 5% (w/v) agarose gel equivalent.
NTSYS software, version 2.02, was used to estimate genetic similarities using Jaccards algorithm while the dendrogram was constructed by UPGMA and Neighbor Joining methods.
High genetic similarity between the samples collected from the agricultural area of Pella, and the samples collected from the Institute of Pomology of Naoussa (>0.70), suggests that they might be clones of some cultivars.
The Greek cultivar Ermioni showed high genetic similarity (95%) with cultivar Wonderful (95%). RAPD and ISSR molecular markers were useful for studying the genetic relationships between pomegranate genotypes and cultivars, with the ISSR markers generating more polymorphic bands.
Authors
M. Pitsiouni, A. Linos, M. Hagidimitriou, P. Drogoudi
Keywords
RAPDs, ISSRs, DNA polymorphism, PCR, accessions
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