Articles
ALPHA CINNAMOMIN ELICITS A DEFENCE RESPONSE AGAINST PHYTOPHTHORA CINNAMOMI IN CASTANEA SATIVA
Article number
940_45
Pages
315 – 321
Language
English
Abstract
Phytophthora cinnamomi and P. cambivora are considered as the causal agents of Castanea sativa ink disease.
These soil-borne plant pathogens invade and destroy the root system leading to the death of the trees.
Most Phytophthora species secrete elicitins, a group of unique highly conserved proteins that are able to enhance plant defence responses in a systemic acquired resistance manner against infection by several pathogens.
A cluster of four elicitin genes was identified in P. cinnamomi. In previous works one of these elicitins, α-cinnamomin was shown to restrict the invasion of root cortical tissues by P. cinnamomi preventing vascular colonization in cork and holm oak.
In the present work, roots of chestnut plantlets grown in vitro were allowed to absorb α-cinnamomin at 100 µg/ml for two days before being inoculated with P. cinnamomi. The effects of this elicitin on host-pathogen interaction were studied at histological and ultra-structural levels.
P. cinnamomi was restricted to the outer cortex of 65% of the roots pre-treated with α-cinnamomin.
In these roots, the vascular cylinders were free of pathogen.
On the contrary, the pathogen reached the vascular cylinder, penetrating the phloem and xylem vessels in all non-treated assayed roots.
The signs of pathogen degradation in the cortical parenchyma, mainly in the intercellular spaces, and the increase of a physical barrier in epidermal and sub-epidermal cell wall-media lamella and intercellular spaces by impregnation with phenol-like compounds strongly suggest that α-cinnamomin induced in chestnut defence reactions against P. cinnamomi.
These soil-borne plant pathogens invade and destroy the root system leading to the death of the trees.
Most Phytophthora species secrete elicitins, a group of unique highly conserved proteins that are able to enhance plant defence responses in a systemic acquired resistance manner against infection by several pathogens.
A cluster of four elicitin genes was identified in P. cinnamomi. In previous works one of these elicitins, α-cinnamomin was shown to restrict the invasion of root cortical tissues by P. cinnamomi preventing vascular colonization in cork and holm oak.
In the present work, roots of chestnut plantlets grown in vitro were allowed to absorb α-cinnamomin at 100 µg/ml for two days before being inoculated with P. cinnamomi. The effects of this elicitin on host-pathogen interaction were studied at histological and ultra-structural levels.
P. cinnamomi was restricted to the outer cortex of 65% of the roots pre-treated with α-cinnamomin.
In these roots, the vascular cylinders were free of pathogen.
On the contrary, the pathogen reached the vascular cylinder, penetrating the phloem and xylem vessels in all non-treated assayed roots.
The signs of pathogen degradation in the cortical parenchyma, mainly in the intercellular spaces, and the increase of a physical barrier in epidermal and sub-epidermal cell wall-media lamella and intercellular spaces by impregnation with phenol-like compounds strongly suggest that α-cinnamomin induced in chestnut defence reactions against P. cinnamomi.
Authors
C. Medeira, I. Maia, C. Ribeiro, I. Candeias, E. Melo, N. Sousa, A. Cravador
Keywords
elicitin, resistance, ink disease, chestnut, pathogen defence, oomycete, biocontrol
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