Articles
ROOTING OF MICROPROPAGATED TRANSSEXUAL PISTACIA TEREBINTHUS L. PLANTS FROM BULGARIA
Article number
940_39
Pages
275 – 281
Language
English
Abstract
The Pistacia terebinthus L. trees demonstrate exceptional drought resistance of the kind which allows cultivation in small-productive, stony and sliding (pliant to erosion) soils.
Biotechnological approaches for preservation, multiplication and inclusion in selection programs of the rare transsexual P. terebinthus L. form found in the Rhodopes Mountain (Bulgaria) are under way.
The aim of the present research is to improve the rooting of micropropagated plants of this form.
The experiments for optimization of the rooting are made with in vitro propagated plants from a single genotype, obtained from an embryo culture.
The plant material is maintained on a solidified DKW nutrient medium supplemented with 2.5 µM BAP, 0.005 µM IBA and 30 g L-1 glucose.
The shootlets obtained during multiplication are elongated on hormone-free DKW or WPM nutrient medium for 10 days in glass jars or in polypropylene vessels (PP) with gas-permeating cover.
The nutrient media for root induction are based on DKW or WPM salt compositions (50% macro-elements) and contain varying auxin concentrations – 0, 10 or 25 µM IBA, combination of 10 µM IBA and 0.054 µM NAA or 10 µM IBA and 0.5 µM IAA. Agar solidified and liquid media with perlite as supporting material are tested.
The percent of rooted plants is evaluated after 25 days.
All plants (rooted and non-rooted in vitro) are potted in a peat-perlite mixture for acclimatization.
At this stage a lot of non-rooted plants develop roots.
Plants survival is evaluated after 50 days.
The highest rooting percent in vitro (76.6%) and the best survival rate after acclimatization (93.3%) is obtained from plants, cultivated on WPM liquid medium with perlite as supporting material, supplemented with 10 µM IBA and 0.5 µM IAA.
Biotechnological approaches for preservation, multiplication and inclusion in selection programs of the rare transsexual P. terebinthus L. form found in the Rhodopes Mountain (Bulgaria) are under way.
The aim of the present research is to improve the rooting of micropropagated plants of this form.
The experiments for optimization of the rooting are made with in vitro propagated plants from a single genotype, obtained from an embryo culture.
The plant material is maintained on a solidified DKW nutrient medium supplemented with 2.5 µM BAP, 0.005 µM IBA and 30 g L-1 glucose.
The shootlets obtained during multiplication are elongated on hormone-free DKW or WPM nutrient medium for 10 days in glass jars or in polypropylene vessels (PP) with gas-permeating cover.
The nutrient media for root induction are based on DKW or WPM salt compositions (50% macro-elements) and contain varying auxin concentrations – 0, 10 or 25 µM IBA, combination of 10 µM IBA and 0.054 µM NAA or 10 µM IBA and 0.5 µM IAA. Agar solidified and liquid media with perlite as supporting material are tested.
The percent of rooted plants is evaluated after 25 days.
All plants (rooted and non-rooted in vitro) are potted in a peat-perlite mixture for acclimatization.
At this stage a lot of non-rooted plants develop roots.
Plants survival is evaluated after 50 days.
The highest rooting percent in vitro (76.6%) and the best survival rate after acclimatization (93.3%) is obtained from plants, cultivated on WPM liquid medium with perlite as supporting material, supplemented with 10 µM IBA and 0.5 µM IAA.
Authors
L.R. Nacheva, P.S. Gercheva, A.T. Zhivondov
Keywords
rooting, pistacia, auxin
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