Articles
GENETIC DIVERSITY AND EPIDEMIOLOGICAL STUDY OF SOME MEDITERRANEAN CTV POPULATIONS
Article number
940_94
Pages
681 – 685
Language
English
Abstract
Citrus tristeza closterovirus (CTV), an insect-borne virus transmitted in nature by several aphid species in a semi-persistent manner, has historically posed a serious threat to the citrus industry worldwide.
Up to date, the virus characterization and virus-vector relationship have been a key factor in understanding the disease epidemiology.
The present research investigated the genetic variability generated within specific genomic regions of selected CTV isolates throughout the transmission by aphids.
Isolates showing different genotype profiles and originating from Italy and Egypt were subjected to experimental transmission trials by a local biotype of Aphis gossypii (Glover) species, onto Mexican lime [Citrus aurantifolia (Christm.) Swingle] seedlings.
The genotypes belonging to these CTV isolates were studied by using simultaneously multiple molecular markers (MMM) analysis, partial genome sequencing on CPg and single strand conformation polymorphism (SSCP) assays flanking the p18 and p20 genomic regions.
The SSCP patterns obtained from the different sub-isolates were compared to those associated with their parental inoculum sources.
In the present work, screening of slight CTV genomic variations in the aphid derived sub-isolates indicated variable changes in the studied proteins, regardless of the transmission abilities of the CTV isolates.
Up to date, the virus characterization and virus-vector relationship have been a key factor in understanding the disease epidemiology.
The present research investigated the genetic variability generated within specific genomic regions of selected CTV isolates throughout the transmission by aphids.
Isolates showing different genotype profiles and originating from Italy and Egypt were subjected to experimental transmission trials by a local biotype of Aphis gossypii (Glover) species, onto Mexican lime [Citrus aurantifolia (Christm.) Swingle] seedlings.
The genotypes belonging to these CTV isolates were studied by using simultaneously multiple molecular markers (MMM) analysis, partial genome sequencing on CPg and single strand conformation polymorphism (SSCP) assays flanking the p18 and p20 genomic regions.
The SSCP patterns obtained from the different sub-isolates were compared to those associated with their parental inoculum sources.
In the present work, screening of slight CTV genomic variations in the aphid derived sub-isolates indicated variable changes in the studied proteins, regardless of the transmission abilities of the CTV isolates.
Authors
D. Yahiaoui, K. Djelouah, A.M. D’Onghia, A. Catara
Keywords
CTV diversity, citrus, epidemiology, aphid vectors, Mediterranean
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