Articles
Molecular phylogeny and biodiversity of the genus Etlingera in Thailand, except the Malay Peninsula region
Article number
1455_26
Pages
219 – 228
Language
English
Abstract
Etlingera is a genus of the Zingiberaceae family that is distributed in Southeast Asia.
This genus has been used for food, spices, and folk medicine in Thailand.
Various morphological characters were confused into the species for plant material utilization.
The aim of this study was to investigate molecular phylogeny and tested DNA barcode efficiency to identify the genus Etlingera in Thailand.
Twenty-one Etlingera specimens were collected from Thailand, except the Malay Peninsula of the southern region.
The internal transcribed spacer (ITS), ribulose-1,5 bisphosphate carboxylase (rbcL), and ribosomal protein S16 (rps16) sequence were used for the study.
The maximum likelihood tree based on ITS locus divided into 5 species as follows: E. araneosa, E. linguiformis, E. littoralis, E. pavieana subsp. hirticalyx, and E. yunnanensis. The rbcL and rps16 can be identified in only one of Etlingera species per locus as E. linguiformis and E. pavieana subsp. hirticalyx, respectively.
The ITS phylogeny revealed the relationships of Thai Etlingera as polytomy, which did not relate to the morphology.
The E. araneosa and E. yunnanensis were more closely related than other species.
In addition, E. araneosa showed a high species population in northern and western of Thailand; whereas, E. littoralis was found in over-estimated area.
Interestingly, the highest biodiversity was exhibited in only the Phetchabun province where three species were found (E. araneosa, E. littoralis, and E. yunnanensis). This study indicated that the ITS marker has a high efficiency as DNA barcode for identifying Etlingera species in Thailand.
This genus has been used for food, spices, and folk medicine in Thailand.
Various morphological characters were confused into the species for plant material utilization.
The aim of this study was to investigate molecular phylogeny and tested DNA barcode efficiency to identify the genus Etlingera in Thailand.
Twenty-one Etlingera specimens were collected from Thailand, except the Malay Peninsula of the southern region.
The internal transcribed spacer (ITS), ribulose-1,5 bisphosphate carboxylase (rbcL), and ribosomal protein S16 (rps16) sequence were used for the study.
The maximum likelihood tree based on ITS locus divided into 5 species as follows: E. araneosa, E. linguiformis, E. littoralis, E. pavieana subsp. hirticalyx, and E. yunnanensis. The rbcL and rps16 can be identified in only one of Etlingera species per locus as E. linguiformis and E. pavieana subsp. hirticalyx, respectively.
The ITS phylogeny revealed the relationships of Thai Etlingera as polytomy, which did not relate to the morphology.
The E. araneosa and E. yunnanensis were more closely related than other species.
In addition, E. araneosa showed a high species population in northern and western of Thailand; whereas, E. littoralis was found in over-estimated area.
Interestingly, the highest biodiversity was exhibited in only the Phetchabun province where three species were found (E. araneosa, E. littoralis, and E. yunnanensis). This study indicated that the ITS marker has a high efficiency as DNA barcode for identifying Etlingera species in Thailand.
Authors
T. Luangsuphabool, A. Wongpia, C. Punvittayagul, K. Pipithsangchan, K. Thammasiri
Keywords
Zingiberaceae, DNA barcodes, molecular diversity
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