Articles
Micropropagation of Psidium cattleyanum, a species native to the Brazilian Cerrado
Article number
1455_37
Pages
313 – 320
Language
English
Abstract
Psidium cattleyanum Sabine (Myrtaceae), commonly known as yellow araçá, is a species native to Brazil, valued for its edible fruits and its ornamental potential.
However, the species faces threats due to the destruction of its natural habitat, the Cerrado.
In this context, in vitro micropropagation emerges as a crucial tool for conservation and a large-scale production of seedlings, in addition to enabling studies on their growth and development under controlled conditions.
This study aimed to develop a protocol for in vitro micropropagation of the species P. cattleyanum by evaluating the response of shoot apices and nodal segments cultivated in MS medium supplemented with different concentrations of BAP (1.0 and 2.0 mg L‑1) and the combination of 1.5 mg L‑1 BAP + 0.5 mg L‑1 IAA, in addition to a control without plant growth regulators.
The variable fresh weight and dry weight, number of leaves, and shoot length were obtained after eight weeks of in vitro culture.
The results demonstrated significant differences between treatments and explant types.
Apices showed better response with the BAP+IAA combination in relation to biomass.
In nodal segments, the highest length and number of shoots were observed in the presence of 2 mg L‑1 BAP; while, the BAP+IAA combination induced callus formation.
This study identified concentrations of plant growth regulators that stimulate in vitro growth of shoot and nodal segments of P. cattleyanum. These results contribute to the development of an efficient micropropagation protocol for this native species.
However, the species faces threats due to the destruction of its natural habitat, the Cerrado.
In this context, in vitro micropropagation emerges as a crucial tool for conservation and a large-scale production of seedlings, in addition to enabling studies on their growth and development under controlled conditions.
This study aimed to develop a protocol for in vitro micropropagation of the species P. cattleyanum by evaluating the response of shoot apices and nodal segments cultivated in MS medium supplemented with different concentrations of BAP (1.0 and 2.0 mg L‑1) and the combination of 1.5 mg L‑1 BAP + 0.5 mg L‑1 IAA, in addition to a control without plant growth regulators.
The variable fresh weight and dry weight, number of leaves, and shoot length were obtained after eight weeks of in vitro culture.
The results demonstrated significant differences between treatments and explant types.
Apices showed better response with the BAP+IAA combination in relation to biomass.
In nodal segments, the highest length and number of shoots were observed in the presence of 2 mg L‑1 BAP; while, the BAP+IAA combination induced callus formation.
This study identified concentrations of plant growth regulators that stimulate in vitro growth of shoot and nodal segments of P. cattleyanum. These results contribute to the development of an efficient micropropagation protocol for this native species.
Authors
R. Paiva, H.A.M. Londono, P.D.O. Paiva
Keywords
plant tissue culture, native species, in vitro growth, 6-benzylaminopurine
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