Articles
Essential oil components and antioxidant activity of Zingiber ottensii Valeton from four regions of Thailand
Article number
1455_27
Pages
229 – 240
Language
English
Abstract
The members of the Zingiberaceae family are an excellent resource for finding potent bioactive compounds, which will be applied to the drug discovery and therapeutic treatment. Zingiber ottensii Valeton is one of notable medicinal plants that have been used for centuries as folk medicines by local Thai communities.
Nevertheless, there are few reports on the study of phytoconstituents and bioactivities of Z. ottensii cultivated in Thailand.
Hence, from nine cultivars of Z. ottensii collected from four geographic locations were extracted the rhizome oils before being subjected to gas chromatography-mass spectrometry (GC-MS). Twenty-seven compounds, corresponding to 88.52-94.09% of volatile oils, were identified.
The major constituents were zerumbone (36.72-38.83%), terpinene-4-ol (6.99-15.93%), sabinene (6.84-17.22%),
-humulene (6.47-11.60%), and 
-pinene (3.77-6.15%). In the ‘Pattani-1’ cultivar the highest percentage of zerumbone was detected, a multifunctional compound that exhibits various pharmacological activities.
Furthermore, total phenolic and flavonoid contents (TPC and TFC) in the ethanolic crude extracts of Z. ottensii’s rhizomes were measured.
The results showed the wide ranges of TPC and TFC depended on individual cultivars (10.01-70.33 mg GAE g‑1 and 1.37-11.15 mg CE g‑1, respectively). Also, antioxidant activities of rhizome extracts were determined using DPPH (2,2-diphenyl-l-picrylhydrazyl), ABTS (2,2’-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt), and FRAP (ferric reducing antioxidant power) assays.
Three methods indicated that nine Z. ottensii cultivars possessed different capacities of DPPH and ABTS radical-scavenging (IC50 3.85-50 and 4.47-50 mg mL‑1), as well as ferric reducing power (14.90-97.83 mg g‑1). ‘Tak-1’ cultivar exhibited the highest antioxidant activities from three assays which correlated to the great amounts of TPC and TFC superior to other cultivars.
The information obtained from this study will not only support the therapeutic properties in traditional uses; it might also promote Z. ottensii utilization for modern pharmacotherapy.
Nevertheless, there are few reports on the study of phytoconstituents and bioactivities of Z. ottensii cultivated in Thailand.
Hence, from nine cultivars of Z. ottensii collected from four geographic locations were extracted the rhizome oils before being subjected to gas chromatography-mass spectrometry (GC-MS). Twenty-seven compounds, corresponding to 88.52-94.09% of volatile oils, were identified.
The major constituents were zerumbone (36.72-38.83%), terpinene-4-ol (6.99-15.93%), sabinene (6.84-17.22%),
-humulene (6.47-11.60%), and -pinene (3.77-6.15%). In the ‘Pattani-1’ cultivar the highest percentage of zerumbone was detected, a multifunctional compound that exhibits various pharmacological activities.Furthermore, total phenolic and flavonoid contents (TPC and TFC) in the ethanolic crude extracts of Z. ottensii’s rhizomes were measured.
The results showed the wide ranges of TPC and TFC depended on individual cultivars (10.01-70.33 mg GAE g‑1 and 1.37-11.15 mg CE g‑1, respectively). Also, antioxidant activities of rhizome extracts were determined using DPPH (2,2-diphenyl-l-picrylhydrazyl), ABTS (2,2’-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt), and FRAP (ferric reducing antioxidant power) assays.
Three methods indicated that nine Z. ottensii cultivars possessed different capacities of DPPH and ABTS radical-scavenging (IC50 3.85-50 and 4.47-50 mg mL‑1), as well as ferric reducing power (14.90-97.83 mg g‑1). ‘Tak-1’ cultivar exhibited the highest antioxidant activities from three assays which correlated to the great amounts of TPC and TFC superior to other cultivars.
The information obtained from this study will not only support the therapeutic properties in traditional uses; it might also promote Z. ottensii utilization for modern pharmacotherapy.
Authors
A. Wongpia, P. Kongsuwan, C. Punvittayagul, T. Luangsuphabool, K. Pipithsangchan, K. Thammasiri
Keywords
Zingiberaceae, medicinal plant, gas chromatography-mass spectrometry, zerumbone, biological activities
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