Articles
In vitro cultivation characteristics of promising Chaenomeles japonica (Thunb.) Lindl. ex Spach cultivars
Article number
1455_30
Pages
253 – 260
Language
English
Abstract
The article presents the results of a study aimed to optimize clonal micropropagation stages of promising cultivars of Chaenomeles japonica (Thunb.) Lindl. ex Spach.
During the in vitro culture initiation stage, one-year-old shoot segments with two nodes were identified as the most effective primary explants.
The highest percentage of viable explants (79-89%) was achieved using a stepwise surface sterilization protocol involving treatment with 2% difenoconazole for 10 min, followed by 70% ethanol for 1 min, and 5% sodium hypochlorite for 3-6 min.
During the multiplication stage, the effects of various plant growth regulators (PGRs): 6-benzylaminopurine (6-BAP), meta-Topolin (mT), and thidiazuron (TDZ) at a concentration of 0.5 mg L‑1, as well as the combinations of mT and 6-BAP at concentrations of 0.15, 0.25, and 0.5 mg L‑1 each, were evaluated for their influence on the regenerative potential of the studied C. japonica cultivars.
It was established that cultivation on Murashige and Skoog (1962) medium supplemented with 0.5 mg L‑1 mT stimulated the activation of axillary buds and the active formation of adventitious microshoots in the basal part of the explant.
This led to a 1.3- to 1.8-fold increase in the multiplication rate (7.4±0.9) compared to the use of 0.5 mg L‑1 6-BAP (5.6±0.7) and 0.5 mg L‑1 TDZ (3.9±0.6). In contrast, the use of TDZ at 0.5 mg L‑1 was ineffective and caused the development of several morphological abnormalities in the regenerants.
The combination of mT and 6-BAP enhanced the regenerative potential of C. japonica cultivars, with the best response observed at a concentration of 0.25 mg L‑1 for each growth regulator, resulting in a multiplication rate of 10.2±1.1. Additionally, it was observed that the studied C. japonica cultivars showed differences in their morphogenetic potential.
During the in vitro culture initiation stage, one-year-old shoot segments with two nodes were identified as the most effective primary explants.
The highest percentage of viable explants (79-89%) was achieved using a stepwise surface sterilization protocol involving treatment with 2% difenoconazole for 10 min, followed by 70% ethanol for 1 min, and 5% sodium hypochlorite for 3-6 min.
During the multiplication stage, the effects of various plant growth regulators (PGRs): 6-benzylaminopurine (6-BAP), meta-Topolin (mT), and thidiazuron (TDZ) at a concentration of 0.5 mg L‑1, as well as the combinations of mT and 6-BAP at concentrations of 0.15, 0.25, and 0.5 mg L‑1 each, were evaluated for their influence on the regenerative potential of the studied C. japonica cultivars.
It was established that cultivation on Murashige and Skoog (1962) medium supplemented with 0.5 mg L‑1 mT stimulated the activation of axillary buds and the active formation of adventitious microshoots in the basal part of the explant.
This led to a 1.3- to 1.8-fold increase in the multiplication rate (7.4±0.9) compared to the use of 0.5 mg L‑1 6-BAP (5.6±0.7) and 0.5 mg L‑1 TDZ (3.9±0.6). In contrast, the use of TDZ at 0.5 mg L‑1 was ineffective and caused the development of several morphological abnormalities in the regenerants.
The combination of mT and 6-BAP enhanced the regenerative potential of C. japonica cultivars, with the best response observed at a concentration of 0.25 mg L‑1 for each growth regulator, resulting in a multiplication rate of 10.2±1.1. Additionally, it was observed that the studied C. japonica cultivars showed differences in their morphogenetic potential.
Authors
O.I. Molkanova, I.L. Krakhmaleva, E.N. Raeva-Bogoslovskaya, I.V. Mitrofanova
Keywords
Japanese quince, clonal micropropagation, culture initiation, growth regulators, multiplication rate
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