Articles
In vitro propagation of Huperzia carinata (Desv.) Trevis., a medicinal plant with potential for Alzheimer’s disease treatment
Article number
1455_39
Pages
329 – 336
Language
English
Abstract
Huperzia carinata (Desv.) Trevis., a medicinal plant rich in Huperzine A (HupA), is a crucial source for Alzheimer’s disease treatment.
However, its slow growth and overharvesting from natural populations threaten its sustainability.
This study aimed to develop an efficient in vitro propagation protocol for H. carinata to support conservation and sustainable HupA production.
Shoot tips, middle stem, and basal stem segments were cultured on semi-solid half-strength Murashige and Skoog (½MS) medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D; 0-2.0 mg L‑1) and 6-benzylaminopurine (BAP; 0-0.5 mg L‑1) for 12 months.
The results showed that basal stem segments were the most responsive explants.
The highest shoot regeneration rate was 6.75±3.15 shoots explant‑1 on ½MS medium supplemented with 0.1 mg L‑1 2,4-D. For rooting, the highest number of roots was 8.00±2.45 roots explant‑1 on ½MS medium supplemented with 2.0 mg L‑1 2,4-D. Additionally, the maximum biomass increase was 4005.13% on the same medium.
This optimized protocol using basal stem segments provides a promising foundation for the large-scale propagation of H. carinata, ensuring a sustainable supply of this valuable medicinal plant and supporting its conservation.
However, its slow growth and overharvesting from natural populations threaten its sustainability.
This study aimed to develop an efficient in vitro propagation protocol for H. carinata to support conservation and sustainable HupA production.
Shoot tips, middle stem, and basal stem segments were cultured on semi-solid half-strength Murashige and Skoog (½MS) medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D; 0-2.0 mg L‑1) and 6-benzylaminopurine (BAP; 0-0.5 mg L‑1) for 12 months.
The results showed that basal stem segments were the most responsive explants.
The highest shoot regeneration rate was 6.75±3.15 shoots explant‑1 on ½MS medium supplemented with 0.1 mg L‑1 2,4-D. For rooting, the highest number of roots was 8.00±2.45 roots explant‑1 on ½MS medium supplemented with 2.0 mg L‑1 2,4-D. Additionally, the maximum biomass increase was 4005.13% on the same medium.
This optimized protocol using basal stem segments provides a promising foundation for the large-scale propagation of H. carinata, ensuring a sustainable supply of this valuable medicinal plant and supporting its conservation.
Authors
S. Rodpradit, P. Klaharn, P. Pumisutapon, T. Paobuathong
Keywords
auxin, club moss, cytokinin, explant types, micropropagation
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