Articles
LEVELS OF 1-AMINOCYCLOPROPANE-1-CARBOXYLIC ACID (ACC) SYNTHASE ACTIVITY, ACC AND ACC-CONJUGATE IN CUT CARNATION FLOWERS DURING SENESCENCE
Article number
181_11
Pages
99 – 104
Language
Abstract
Accompanying the climacteric rise in ethylene production associated with senescence in cut carnation flowers (Dianthus caryophyllus L. cv.
White Sim) were increases in ACC synthase activity and contents of ACC and conjugate-ACC. Comparing ACC synthase activity in the petals with the remaining flower parts, activity began to rise sooner in the latter.
The increase in level of ACC in the petals and remaining flower parts correlated with the increase in ACC synthase activity in the two tissues.
The level of conjugate-ACC was about 10 times greater than that of ACC in pre-climacteric flowers (ACC content at this stage was 0.2–0.4 nmoles/g fresh weight). At the post-climacteric stage the level of conjugate-ACC had increased almost 8-fold that of the pre-climacteric level.
Based on thin-layer chromatography the conjugate-ACC was N-malonyl-ACC.
White Sim) were increases in ACC synthase activity and contents of ACC and conjugate-ACC. Comparing ACC synthase activity in the petals with the remaining flower parts, activity began to rise sooner in the latter.
The increase in level of ACC in the petals and remaining flower parts correlated with the increase in ACC synthase activity in the two tissues.
The level of conjugate-ACC was about 10 times greater than that of ACC in pre-climacteric flowers (ACC content at this stage was 0.2–0.4 nmoles/g fresh weight). At the post-climacteric stage the level of conjugate-ACC had increased almost 8-fold that of the pre-climacteric level.
Based on thin-layer chromatography the conjugate-ACC was N-malonyl-ACC.
Authors
G. Peiser
Keywords
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