Articles
METABOLIC CHANGES DURING CYANAMIDE INDUCED DORMANCY RELEASE IN GRAPEVINES
Article number
329_63
Pages
271 – 274
Language
Abstract
Delayed and ununiform bud break is a major problem for grapevines in warm climates, leading to highly vegetative unfruitful vineyards.
A system using single bud cuttings was developed to determine the depth of vine dormancy.
Grapevines were found considerably less responsive to dormancy breaking chemicals than most deciduous fruit trees.
Specific agents were developed for use on grapevines in regions with warm winters.
H2CN2 is one of those chemicals which was found most potent for breaking grapevine dormancy and even at higher efficiency than in many other species.
Catalase activity was shown to drop significantly during chilling.
In this study we could show that most dormancy breaking chemicals such as H2CN2, some of its analogues and a few other agents inhibited catalase activity in accordance with their activity in breaking dormancy.
However, Aminotriazol which was potent in catalase inhibition was not active in inducing bud break, probably due to its additional inhibition later in the pathway.
We found at the same time an accumulation of H2O2 which was accompanied with an increase of peroxidase activity, probably due to substrate adaptability.
It is suggested that this change in the oxidative level of the tissue leads to activation of the pentose phosphate pathway and to growth initiation.
Presently specific changes in the content of various proteins were found at different stages of the dormant period.
A system using single bud cuttings was developed to determine the depth of vine dormancy.
Grapevines were found considerably less responsive to dormancy breaking chemicals than most deciduous fruit trees.
Specific agents were developed for use on grapevines in regions with warm winters.
H2CN2 is one of those chemicals which was found most potent for breaking grapevine dormancy and even at higher efficiency than in many other species.
Catalase activity was shown to drop significantly during chilling.
In this study we could show that most dormancy breaking chemicals such as H2CN2, some of its analogues and a few other agents inhibited catalase activity in accordance with their activity in breaking dormancy.
However, Aminotriazol which was potent in catalase inhibition was not active in inducing bud break, probably due to its additional inhibition later in the pathway.
We found at the same time an accumulation of H2O2 which was accompanied with an increase of peroxidase activity, probably due to substrate adaptability.
It is suggested that this change in the oxidative level of the tissue leads to activation of the pentose phosphate pathway and to growth initiation.
Presently specific changes in the content of various proteins were found at different stages of the dormant period.
Authors
G. Nir, S. Lavee
Keywords
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