Articles
“IN VITRO” FERTILIZATION OF PLUM
Article number
359_41
Pages
269 – 277
Language
Abstract
Fertilization "in vitro" of plums was performed in two stages.
First stage was carried out one week before flowering in orchard.
The flowers of two cultivars: Sweet Common Prune and Stanley were accelerated in the growth room.
The ovules of Sweet Common Prune were pollinated with pollen of Stanley.
Second stage was carried out in reciprocal crosses on flowers of both cultivars collected at the first, second and fourth day of flowering time in orchard.
In both stages the plant material was sterilized according to established method: 1 % sodium hypochloride (2,5 min for ovaries and 1 min for anthers). Excised ovules were placed on White medium with 15 % of sucrose and pollinated with pollen extracted from anthers.
The percentage of germinating pollen grains, chemotropism of pollen tubes and percentage of ovules with pollen tubes penetrating micropyle were estimated 48 hours after pollination.
The ovules designed for further experiments were transfered onto two media: White with 5 % of sucrose or MS + 0,5 mg/dm3 GA3 with 2 % of sucrose and one part was placed in darkness while the second part was exposed to light.
The following observations were done after another 7 days: percentage of growing embryos, percentage of objects growing into callose, percentage of vanishing objects, and percentage of objects with no reaction.
The
First stage was carried out one week before flowering in orchard.
The flowers of two cultivars: Sweet Common Prune and Stanley were accelerated in the growth room.
The ovules of Sweet Common Prune were pollinated with pollen of Stanley.
Second stage was carried out in reciprocal crosses on flowers of both cultivars collected at the first, second and fourth day of flowering time in orchard.
In both stages the plant material was sterilized according to established method: 1 % sodium hypochloride (2,5 min for ovaries and 1 min for anthers). Excised ovules were placed on White medium with 15 % of sucrose and pollinated with pollen extracted from anthers.
The percentage of germinating pollen grains, chemotropism of pollen tubes and percentage of ovules with pollen tubes penetrating micropyle were estimated 48 hours after pollination.
The ovules designed for further experiments were transfered onto two media: White with 5 % of sucrose or MS + 0,5 mg/dm3 GA3 with 2 % of sucrose and one part was placed in darkness while the second part was exposed to light.
The following observations were done after another 7 days: percentage of growing embryos, percentage of objects growing into callose, percentage of vanishing objects, and percentage of objects with no reaction.
The
Authors
W. Lech, M. Malodobry, M. Lech, B. Nowak
Keywords
chemotropism, fertilization, in vitro, Prunus domestica, plum
Online Articles (43)