ISOLATION AND IDENTIFICATION OF INDUCED SECONDARY METABOLITES FROM CELL SUSPENSION CULTURES OF FAGUS SYLVATICA

Cell suspension cultures of Fagus sylvatica were used to study the induction of secondary phenolic metabolites after treatment with elicitors originating from Sclerotium rolfsii and Phytophthora megasperma f.sp. glycinea, chitosan as well as with UV/visible radiation.
Induced metabolites were isolated and their structures elucidated on the basis of chemical and spectroscopic (fluorescence, UV, FT-IR, MS, NMR) techniques.
Two soluble compounds were identified as 4-(acetoxymethyl)phenyl- -D-glucopyranoside and N-(4-coumaroyl)-aspartate.
Mild alkaline hydrolysis of cell wall material released three metabolites, which were characterized as 4-hydroxybenzaldehyde, vanillin and 4-coumaric acid. N-(4-Coumaroyl)-aspartate was a major metabolite after elicitor treatment, while other compunds of as yet unknown structure were induced after UV/visible irradiation.
The fact, that elicitors and UV/visible treatment induced different metabolic pathways in the cell suspension cultures suggests, that secondary metabolites may be useful as specific biomarkers for environmental stress.

International Symposium on Natural Phenols in Plant Resistance

T.P. Jungblut, W. Heller, N. Hertkorn, J. Lintelmann, H. Sandermann Jr

Fagus sylvatica, cell cultures, phenylpropanoids, hydroxycinnamic acids, N-(E-4-coumaroyl)aspartate, hydroxybenzaldehydes

https://doi.org/10.17660/ActaHortic.1994.381.60