Articles

THE ACTIVITY OF PHENYLALANINE AMMONIA-LYASE IN APPLE LEAVES AFTER WOUNDING

Article number
381_61
Pages
474 – 478
Language
Abstract
A spectrophotometric method for measuring the activity of phenylalanine ammonia-lyase (PAL, E.C. 4.3.1.5) was adapted to apple leaves.
The enzyme is characterized by optima of pH (8.2) and temperature (55°C) and showed its highest activity in the young leaves, levelling down to 20% from the first to the fourth leaf.
Pressure treatment of the leaf discs enhanced the enzyme activity in the light with a peak after 24 hours.
The fact that soluble phenols accumulated later in treated discs than in untreated ones is discussed with reference to metabolic and catabolic processes.

Publication
Authors
S. Fünfgelder, U. Mayr, D. Treutter, W. Feucht
Keywords
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