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Articles

TOTAL PHENOLS AND PHYTOALEXINS ACCUMULATION IN COFFEE LEAVES INFECTED WITH HEMILEIA VASTATRIX BERK ET BR. AND PSEUDOMONAS SYRINGAE

Article number
381_75
Pages
561 – 564
Language
English
Abstract
Two years old plants of Coffea arabica L. were inoculated with the rust Hemileia vastatrix Berk et Br. or infiltrated with a bacterial suspension of Pseudomonas syringae. The presence of biocidal compounds in the extracts was assessed by a bioassay method, with a dense suspension of Cladosporium cucumerinum ELL. et Arth (Allen & Kúc, 1968). The characterization of phytoalexins was made mainly by high-field NMR spectroscopy and mass spectrometry.
The total phenol content was determined in the extract obtained from the acetone powders and calculated as the quantity of chlorogenic acid present.

Publication
Authors
M.E.M. Guedes, L.E. Guerra-Guimaräes, A.M.S. Silva, J. Cavaleiro
Keywords
Full text
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