Articles
IN VITRO SELECTION OF TOMATO PLANTS RESISTANT TO PSEUDOMONAS SOLANACEARUM
Article number
392_13
Pages
115 – 122
Language
Abstract
The toxin responsible for causing wilt disease in tomato was isolated from Pseudomonas solanacearum race 1 biovar 3 by alcohol precipitation and ion exchange chromatography.
Characterization of toxin revealed the presence of glucose, mannose and polypeptides.
Cell lines of 5 wilt susceptible tomato varieties, growing in modified MS medium, were treated with purified toxin (EC50). Cells showing toxin insensitivity were isolated and regenerated to plants.
One regenerated somaclone showed an high level of resistance to P. solanacearum, while two other somaclones showed moderate resistance.
Field tests of the somaclones for 3 years showed segregation of the character.
Resistant plants showed a significant increase in rishitin production following toxin and pathogen treatments.
Toxin was shown to induce physical damage of the membrane system in susceptible cells.
Characterization of toxin revealed the presence of glucose, mannose and polypeptides.
Cell lines of 5 wilt susceptible tomato varieties, growing in modified MS medium, were treated with purified toxin (EC50). Cells showing toxin insensitivity were isolated and regenerated to plants.
One regenerated somaclone showed an high level of resistance to P. solanacearum, while two other somaclones showed moderate resistance.
Field tests of the somaclones for 3 years showed segregation of the character.
Resistant plants showed a significant increase in rishitin production following toxin and pathogen treatments.
Toxin was shown to induce physical damage of the membrane system in susceptible cells.
Authors
S.J.N. Baruah, P.C. Deka
Keywords
Lycopersicon esculentum, Pseudomonas solanacearum, Somaclone, Toxin isolation, Wilt resistance
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