Articles
A ‘SHUTTLE CALLUS SYSTEM’- APPLICATION OF TISSUE CULTURE IN CITRUS
Article number
392_4
Pages
39 – 48
Language
Abstract
Potential applications of tissue culture to conduct basic research and applied studies in citrus are presented.
A ‘shuttle callus system’, in which embryogenic callus obtained from embryos or embryoids can produce embryoids, and the embryoids in turn can produce embryogenic callus reciprocally, is described.
Embryogenic callus could also be produced from anther culture-derived embryoids.
Viable protoplasts could be produced easily from embryogenic callus and used in somatic hybridization and direct DNA transfer.
Studies on cell selection, cell fusion and genetic transformation in this system are also discussed.
A ‘shuttle callus system’, in which embryogenic callus obtained from embryos or embryoids can produce embryoids, and the embryoids in turn can produce embryogenic callus reciprocally, is described.
Embryogenic callus could also be produced from anther culture-derived embryoids.
Viable protoplasts could be produced easily from embryogenic callus and used in somatic hybridization and direct DNA transfer.
Studies on cell selection, cell fusion and genetic transformation in this system are also discussed.
Authors
T. Hidaka
Keywords
Somatic embryogenesis, Cell culture, Protoplast, Cell selection, Cell fusion, Transformation
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