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Articles

AGROBACTERIUM-MEDIATED GENETIC TRANSFORMATION OF CHRYSANTHEMUM

Article number
392_17
Pages
147 – 152
Language
Abstract
Transgenic chrysanthemum (Dendranthema grandiflorum (Ramat.) Kitamura) plants were obtained using Agrobacterium tumefaciens strain AGLO containing the binary vector pMOG410 carrying the neomycine phosphotransferase II and beta-glucuronidase-intron genes.
Genotypic differences in regeneration potential and sensitivity to kanamycin affected the final efficiencies of transformation.
Variations in phenotypical features were observed among clones in which the presence of both genes in their genome was confirmed by PCR.

Publication
Genetic Improvement of Horticultural Crops by Biotechnology
Authors
S. Fukai, J. De Jong, W. Rademaker
Keywords
Agrobacterium tumefaciens, Chrysanthemum, Genetic transformation
Full text
https://doi.org/10.17660/ActaHortic.1995.392.17
Online Articles (32)
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T. Hidaka
A.R. van der Krol | O. Vorst
J.B. Nasrallah
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M. Hagimori
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S.J.N. Baruah | P.C. Deka
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M. Hiramatsu | K. Odahara | Y. Matsue
S. Fukai | J. De Jong | W. Rademaker
C. Suginuma | T. Akihama
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A. da Câmara Machado | E. Knapp | H. Pühringer | G. Seifert | V. Hanzer | H. Weiss | Q. Wang | H. Katinger | M. Laimer da Câmara Machado
S. Kusaba | Y. Kano-Murakami | M. Matsuoka | M. Fukumoto
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R. Aida | M. Shibata
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M.S. Kang | S.G. Suh | I. Maruta | K.W. Kim
S. Hiratsuka | Y. Okada