Articles
FUNCTIONAL ANALYSIS OF B-TYPE FLORAL HOMEOTIC MADSBOX GENES.
Article number
392_5
Pages
49 – 60
Language
Abstract
Recent genetic studies on floral development in both Arabidopsis and Antirrhinum have lead to the identification of floral homeotic genes encoding DNA-binding proteins with a common DNA-binding motif: the MADSbox gene family.
Three different MADSbox-genes have been isolated from Petunia hybrida (pMADS-1, -2 and fbpl) that are expressed in the second and third floral whorl.
This indicates that they may function in petal and stamen development and belong to the B-type of MADSbox genes.
The pMADS-1 gene was shown to be essential for petal development by complementing the petunia mutant green petal in which the endogenous pMADS 1 gene is deleted.
Alternatively, the pMADS 1 gene function was demonstrated by suppressing pMADS 1 expression in wild type petunia’s through co suppression and thus phenocopying the green petal mutant.
Suppression of the fbpl gene activity in wild type petunia lead to homeotic conversion of petal to sepal and stamen to carpet (Angenent et al., 1993), demonstrating the importance of this gene in second and third whorl development.
Several differences between the functioning of B-type MADSbox genes in petunia and Antirrhinuim and Arabidopsis will be discussed as well as differences in phenotype generated by ectopic expression of B-type MADSbox genes in petunia and Arabidopsis.
Three different MADSbox-genes have been isolated from Petunia hybrida (pMADS-1, -2 and fbpl) that are expressed in the second and third floral whorl.
This indicates that they may function in petal and stamen development and belong to the B-type of MADSbox genes.
The pMADS-1 gene was shown to be essential for petal development by complementing the petunia mutant green petal in which the endogenous pMADS 1 gene is deleted.
Alternatively, the pMADS 1 gene function was demonstrated by suppressing pMADS 1 expression in wild type petunia’s through co suppression and thus phenocopying the green petal mutant.
Suppression of the fbpl gene activity in wild type petunia lead to homeotic conversion of petal to sepal and stamen to carpet (Angenent et al., 1993), demonstrating the importance of this gene in second and third whorl development.
Several differences between the functioning of B-type MADSbox genes in petunia and Antirrhinuim and Arabidopsis will be discussed as well as differences in phenotype generated by ectopic expression of B-type MADSbox genes in petunia and Arabidopsis.
Authors
A.R. van der Krol, O. Vorst
Keywords
Flower development, MADSbox genes, Petunia, Antirrhinum, Arabidopsis, Feedback
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