Articles
REGULATION OF GENE EXPRESSION BY 2,4-D DURING ORGANOGENESIS IN GLADIOLUS CV. TOPAZ CALLUS
Article number
392_30
Pages
251 – 256
Language
Abstract
Calli were induced from gladiolus cormel tissue, subcultured on Murashige and Skoog (MS) medium with 0.5mg/l 2,4-D and differentiated to root on 2,4-D free MS medium.
Root formation was observed 6 days after transfer to 2,4-D free MS medium.
Changes of protein patterns and in vitro translation products were observed during root formation by isoelectric focusing-sodium dodecyl sulfate polyacrylamide gel electrophoresis (IEF-SDS·PAGE). Over 300 proteins were separated by IEF-SDS· PAGE and qualitative and quantitative changes were observed in proteins having molecular weights from 26kDa to 84Da.
Three proteins were de novo synthesized 6 days after callus culture for root formation.
Five newly synthesized proteins of low molecular weights were observed in in vitro translation products of mRNA prepared from root-forming callus.
Root formation was observed 6 days after transfer to 2,4-D free MS medium.
Changes of protein patterns and in vitro translation products were observed during root formation by isoelectric focusing-sodium dodecyl sulfate polyacrylamide gel electrophoresis (IEF-SDS·PAGE). Over 300 proteins were separated by IEF-SDS· PAGE and qualitative and quantitative changes were observed in proteins having molecular weights from 26kDa to 84Da.
Three proteins were de novo synthesized 6 days after callus culture for root formation.
Five newly synthesized proteins of low molecular weights were observed in in vitro translation products of mRNA prepared from root-forming callus.
Authors
M.S. Kang, S.G. Suh, I. Maruta, K.W. Kim
Keywords
Gladiolus, Callus, 2,4-D, Organogenesis, mRNA, In vitro translation, IEF-SDS· PAGE
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