Articles
COMPARISON OF PROMOTER-DEPENDENT EXPRESSION OF β-GLUCURONIDASE GENES IN TRANSGENIC TORENIA PLANTS
Article number
392_26
Pages
219 – 226
Language
Abstract
Promoter-dependent expression of introduced 
-glucuronidase (GUS) gene in the transgenic torenia plants (Torenia fournieri) is reported.
GUS chimeric genes used in this experiment were, 1: coding region of GUS gene containing an intron fused with cauliflower mosaic virus-35S (CaMV35S) promoter (35S/Intron-GUS), 2: coding region of GUS gene fused with revised CaMV35S promoter having the omega arrangement derived from tobacco mosaic virus (E12Ω/GUS), and 3: coding region of GUS gene fused with PR1a promoter derived from pathogenesis-related la protein gene of tobacco (PR1a/GUS). These GUS chimeric genes were introduced into torenia plants by Agrobacterium-mediated transformation.
The plants harbouring the E12Ω/GUS showed higher GUS activity than the plants harbouring the 35S/Intron-GUS. All plants harbouring the E12 Ω /GUS showed GUS activity more than 10 nanomoles 4-methylumbelliferone/mg protein/30min. at 37°C. Though only 37 % of the plants harbouring the 35S/Intron-GUS showed the activity of more than 10. The expression level in the plants harbouring the PR1a/GUS was increased markedly by salicylic acid (SA) treatment.
The 17.7 times higher GUS activity was observed in the selected 10 plants harbouring the PR1a/GUS by treatment with 0.5 mM SA for 3 days when compared with that of non-treated.
Usefulness of these promoters for controlling expression of introduced genes was shown in torenia plants, as in the case of other plants.
-glucuronidase (GUS) gene in the transgenic torenia plants (Torenia fournieri) is reported.GUS chimeric genes used in this experiment were, 1: coding region of GUS gene containing an intron fused with cauliflower mosaic virus-35S (CaMV35S) promoter (35S/Intron-GUS), 2: coding region of GUS gene fused with revised CaMV35S promoter having the omega arrangement derived from tobacco mosaic virus (E12Ω/GUS), and 3: coding region of GUS gene fused with PR1a promoter derived from pathogenesis-related la protein gene of tobacco (PR1a/GUS). These GUS chimeric genes were introduced into torenia plants by Agrobacterium-mediated transformation.
The plants harbouring the E12Ω/GUS showed higher GUS activity than the plants harbouring the 35S/Intron-GUS. All plants harbouring the E12 Ω /GUS showed GUS activity more than 10 nanomoles 4-methylumbelliferone/mg protein/30min. at 37°C. Though only 37 % of the plants harbouring the 35S/Intron-GUS showed the activity of more than 10. The expression level in the plants harbouring the PR1a/GUS was increased markedly by salicylic acid (SA) treatment.
The 17.7 times higher GUS activity was observed in the selected 10 plants harbouring the PR1a/GUS by treatment with 0.5 mM SA for 3 days when compared with that of non-treated.
Usefulness of these promoters for controlling expression of introduced genes was shown in torenia plants, as in the case of other plants.
Authors
R. Aida, M. Shibata
Keywords
Torenia fournieri, Cauliflower mosaic virus-35S promoter, Pathogenesis-related protein gene promoter, β-glucuronidase, Genetic transformation, Gene expression, Salicylic acid
Online Articles (32)