Articles
IDENTIFICATION OF A RAPD MARKER CLOSELY LINKED TO THE FRUIT COLOR IN MUSCADINE GRAPES (Vitis rotundifoliaI)
Article number
528_35
Pages
265 – 268
Language
Abstract
The major fruit color phenotypes in grapes are either white (including green, yellow, bronze etc.) or red (including pink, reddish) and black (including purple). Like other fruits, anthocyanin (Acy) pigments in the epidermal cells of fruits contribute to the colors of grapes.
In breeding practice, seedling screening can be performed if a genetic marker for the fruit color is available.
A F1 population of 82 progenies derived from a cross of two muscadine grape (Vitis rotundifolia) cultivars, ‘Summit’ x ‘Noble’, was used for tagging the gene determining the fruit color. ‘Noble’ is a red grape while ‘Summit’ is white.
Segregation of berry color in the F1 population indicated that the red color is controlled by a single dominant gene.
RAPD technique and Bulk Segregant Analysis (BSA) was used for tagging the fruit color trait.
A total of 350 oligonucleotide 10-mers were screened for polymorphisms between the red and white colored DNA pools (each pool was consisted of 7 individual DNA samples). Two RAPD fragments linked to the target gene were identified and one of them, a 650 bp fragment completely co-segregated with the 56 progenies of red berries, while the white fruit progenies were absent of the RAPD fragment.
The result demonstrated that the RAPD fragment is a reliable marker for early selection of fruit color trait.
Tight linkage between the RAPD fragment and the red fruit color may also suggest that a gene influencing the Acy pigments synthesis is ready to be cloned in muscadine grapes.
In breeding practice, seedling screening can be performed if a genetic marker for the fruit color is available.
A F1 population of 82 progenies derived from a cross of two muscadine grape (Vitis rotundifolia) cultivars, ‘Summit’ x ‘Noble’, was used for tagging the gene determining the fruit color. ‘Noble’ is a red grape while ‘Summit’ is white.
Segregation of berry color in the F1 population indicated that the red color is controlled by a single dominant gene.
RAPD technique and Bulk Segregant Analysis (BSA) was used for tagging the fruit color trait.
A total of 350 oligonucleotide 10-mers were screened for polymorphisms between the red and white colored DNA pools (each pool was consisted of 7 individual DNA samples). Two RAPD fragments linked to the target gene were identified and one of them, a 650 bp fragment completely co-segregated with the 56 progenies of red berries, while the white fruit progenies were absent of the RAPD fragment.
The result demonstrated that the RAPD fragment is a reliable marker for early selection of fruit color trait.
Tight linkage between the RAPD fragment and the red fruit color may also suggest that a gene influencing the Acy pigments synthesis is ready to be cloned in muscadine grapes.
Authors
Z. Ren, O. Lamikanra, J. Lu
Keywords
Anthocyanin pigments, bunch grapes, Vitis vinifera, bulk segregant analysis (BSA), linkage map
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