Articles
TRANSFORMATION STUDIES ON VITIS VINIFERA L., VIA AGROBACTERIUM TUMEFACIENS
Article number
528_50
Pages
359 – 360
Language
Abstract
Somatic embryos of Vitis vinifera cv.
DORNFELDER obtained from anthers were transformed via Agrobacterium tumefaciens strain LBA 4404 with a plasmid carrying the genes of
-glucuronidase (GUS) with intron and neomycinphosphotransferase II (NPT). The somatic embryos proliferated in liquid NN69 medium containing kanamycin for selection and cefotaxim for removing the bacteria.
Subsequent cultivation of larger embryos was carried out on solid NN69 medium supplemented with kanamycin.
Transgenic shoots were selected and could be converted into rooted plantlets on LS medium.
Transformation of the foreign genes was verified by-glucuronidase assays and PCR amplifications.
DORNFELDER obtained from anthers were transformed via Agrobacterium tumefaciens strain LBA 4404 with a plasmid carrying the genes of
-glucuronidase (GUS) with intron and neomycinphosphotransferase II (NPT). The somatic embryos proliferated in liquid NN69 medium containing kanamycin for selection and cefotaxim for removing the bacteria.Subsequent cultivation of larger embryos was carried out on solid NN69 medium supplemented with kanamycin.
Transgenic shoots were selected and could be converted into rooted plantlets on LS medium.
Transformation of the foreign genes was verified by
-glucuronidase assays and PCR amplifications.
Authors
B.-A. Bornhoff, M. Harst, E. Zyprian, R. Töpfer, C. Iannini
Keywords
Agrobacterium tumefaciens, Vitis vinifera, embryo suspension culture, pH value, transformation
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