Articles

GENETIC TRANSFORMATION OF TOBACCO AND GRAPEVINE FOR RESISTANCE TO VIRUSES RELATED TO THE RUGOSE WOOD DISEASE COMPLEX

Article number
528_44
Pages
323 – 330
Language
Abstract
In the framework of a study aiming at producing transgenic grapevines resistant to viruses of the rugose wood disease complex, the movement protein (MP) genes of Grapevine virus A (GVA) and B (GVB) were analysed in transgenic Nicotiana plants.
The cassettes transcribing MP genes in their sense (MP+) and antisense (MP-) orientation were transferred to Nicotiana benthamiana and N. occidentalis by Agrobacterium tumefaciens-mediated transformation.
Regenerants expressed the RNA transcripts.
R1 seedlings expressing the antisense gene, when challenge-inoculated with the homologous virus showed a consistent reduction of virus titer, and did not show symptoms up to 18 days post inoculation.
MP+ lines showed lower ELISA readings and percentage of symptomatic plants as compared with the non-transgenic control.
Both constructs were successfully inserted in somatic embryos of Vitis rupestris and V. vinifera cv.
Superior seedless, from which several plants were regenerated.

Publication
Authors
L. Martinelli, D. Costa, V. Poletti, S. Festi, N. Buzkan, A. Minafra, P. Saldarelli, G.P. Martelli, A. Perl
Keywords
genetic transformation, movement protein, rugose wood disease complex, Vitis
Full text
Online Articles (115)
M. Boselli | C. Iannini | C. Corso | A. Monaco | D. Iannelli | C. Cottone
A. Costacurta | A. Calò | R. Carraro | C. L. M. Giust
I. Pejic | E. Maletic | J. Karoglan Kontic | B. Kozina | N. Mirosevic
J.M. Martínez-Zapater | J.A. Cabezas | M.T. Cervera
F. Ferreira Monteiro | E. Nunes | R. Magalhaes | M.A. Faria | J.E. Bowers | C.P. Meredith | A. Martins
J.E. Bowers | R. Siret | C.P. Meredith | P. This | J.-M. Boursiquot
U. Malossini | M.S. Grando | I. Roncador | F. Mattivi
S. Faraj | J.L. Cenis | E. Sánchez-Escribano | J.M. Ortiz
F. Regner | A. Stadlhuber | C. Eisenheld | H. Kaserer
M.S. Grando | C. Frisinghelli | M. Stefanini
M.S. Lopes | D. Mendonça | A. da Câmara Machado | M. Laimer da Câmara Machado | H. Steinkellner | K. Sefc
K.D. Scott | L.S. Lee | T. Dow | R.J. Henry
P. This | F. Lahogue | A.F. Adam-Blondon | A. Doligez | A. Bouquet
N.I. Primikirios | F. Lefort | K.A. Roubelakis-Angelakis | K.A. Loulakakis
E.M. Ablett | L.S. Lee | R.J. Henry
M.R. Thomas | P. Iocco | T. Franks
M. Harst | B.-A. Bornhoff | E. Zyprian | R. Töpfer | G. Jach
R. Gölles | R. Moser | H. Pühringer | H. Katinger | M. Laimer da Câmara Machado | A. Minafra | V. Savino | P. Saldarelli | G.P.M. da Câmara Machado
M.C. Mauro | P. Coutos-Thevenot | M. Boulay | L. Valat | P. Barbier | B. Walter | L. Pinck
L. Martinelli | D. Costa | V. Poletti | S. Festi | N. Buzkan | A. Minafra | P. Saldarelli | G.P. Martelli | A. Perl
J.P. Roustan | S. Colrat | S. Dalmayrac | P. Guillen | M. Guis | G. Martinez-Reina | C. Deswarte | M. Bouzayen | J. Fallot | J.C. Pech | A. Latché
B.-A. Bornhoff | M. Harst | E. Zyprian | R. Töpfer | C. Iannini
I.J. Tsvetkov | A.I. Atanassov | V.M. Tsolova
I. Gribaudo | R. Vallania | T. Franks | M.L. Miaja | M. Thomas
A. Matt | R. Blaich | G.M. Reustle
M.C. Heloir | P. Kedziora | J.C. Fournioux | M. Barbier | G. Luquet
P. Cindric | N. Korac | V. Kovac
A. Bouquet | Y. Danglot | M. Bongiovanni | P. Castagnone-Sereno | D. Esmenjaud | A. Dalmasso | L. Torregrosa
S. Gu | K.L. Wilker | M.R. Dharmadhikari | J.F. Moore | S. Howard | C.E. Edson | T.M. Bonney | M.K. Walsh
P. Jeandet | M. Adrian | A.C. Breuil | M. Sbaghi | S. Debord | R. Bessis | L.A. Weston | R. Harmon
É. Sárdi | J. Korbuly | Zs. Királyné Véghely | E. Minsovics
A. Perl | N. Sahar | P. Spiegel-Roy | S. Gavish | R. Elyasi | E. Orr | H. Bazak
L.M. Avramov | D.M. Zunic | B.V. Sivcev | M.M. Ruzevic | N.N. Gasic
M. Milutinovic | D. Nikolic | L. Avramov | V. Rakonjac
E. Garcia | A. Martinez | E. Garcia de la Calera | L.J. Perez | J.L. Cenis | J. Carreño
C.V. Pommer | I.J.A. Ribeiro | I.R.S. Passos | M.M. Terra | E.J.P. Pires | F.P. Martins | L.N. Frutuoso
M. Borgo | G. Ferroni | G. Salvi | G. Scalabrelli
O. Jacquet | M. Laurent | J. Oustric | G. Sanchez | L. Audeguin | L. Lurton | C. Sipp