Articles
TRANSGENIC GRAPEVINES EXPRESSING COAT PROTEIN GENE SEQUENCES OF GRAPEVINE FANLEAF VIRUS, ARABIS MOSAIC VIRUS, GRAPEVINE VIRUS A AND GRAPEVINE VIRUS B
Article number
528_42
Pages
307 – 314
Language
Abstract
Grapevine viruses are of great economic importance because of their worldwide occurrence and the severe damages they cause.
Virus resistance breeding in grapevine is limited since resistance genes either do not exist or are not yet known.
In an attempt to induce virus resistance we introduced nine different constructs containing chimeric coat protein (CP) genes of grapevine fanleaf virus (GFLV), including nontranslatable and truncated forms of the CP gene, arabis mosaic virus (ArMV), grapevine virus A (GVA), and grapevine virus B (GVB) first into the herbaceous host Nicotiana benthamiana and N. occidentalis, respectively, and subsequently into embryogenic cultures of Vitis vinifera (Russalka cv. selfpollinated) and 110 Richter (Vitis rupestris x Vitis Berlandieri) by Agrobacterium-mediated transformation.
Putative transformed tissues were selected by continued proliferation on kanamycin containing medium.
Selected embryos were germinated.
Integration of the different CP genes was confirmed by PCR. Parallel experiments with N. benthamiana, expressing the full-length CP gene of GFLV, showed protection against virus inoculation.
Virus resistance breeding in grapevine is limited since resistance genes either do not exist or are not yet known.
In an attempt to induce virus resistance we introduced nine different constructs containing chimeric coat protein (CP) genes of grapevine fanleaf virus (GFLV), including nontranslatable and truncated forms of the CP gene, arabis mosaic virus (ArMV), grapevine virus A (GVA), and grapevine virus B (GVB) first into the herbaceous host Nicotiana benthamiana and N. occidentalis, respectively, and subsequently into embryogenic cultures of Vitis vinifera (Russalka cv. selfpollinated) and 110 Richter (Vitis rupestris x Vitis Berlandieri) by Agrobacterium-mediated transformation.
Putative transformed tissues were selected by continued proliferation on kanamycin containing medium.
Selected embryos were germinated.
Integration of the different CP genes was confirmed by PCR. Parallel experiments with N. benthamiana, expressing the full-length CP gene of GFLV, showed protection against virus inoculation.
Authors
R. Gölles, R. Moser, H. Pühringer, H. Katinger, M. Laimer da Câmara Machado, A. Minafra, V. Savino, P. Saldarelli, G.P.M. da Câmara Machado
Keywords
Vitis, pathogen-derived resistance, nepovirus, trichovirus, virus resistance
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