Articles

THE USE OF MOLECULAR MARKERS FOR DURABLE RESISTANCE BREEDING IN THE CULTIVATED STRAWBERRY (FRAGARIA x ANANASSA)

Article number
567_132
Pages
615 – 618
Language
English
Abstract
In France, particularly in the southwestern area, most damage to the cultivated strawberry is anthracnose, caused by Colletotrichum acutatum. Anthracnose is a polycyclic disease for which no satisfactory disease control method has been developed.
Therefore, the objective of our project is to construct durable resistance by combining the different genetic components of resistance to lower both the level of damage and the use of chemicals.
Genetic components will be selected by using molecular markers linked to these loci.
For monogenic resistance to the pathogenicity group 2 of C. acutatum, bulk segregant analysis (BSA) combined with the AFLP markers has been developed.
Four markers were found linked to the dominant gene with a recombination percentage ranging from 0 to 13.9%. For complex resistance to the pathogenicity group 1 of C. acutatum, a linkage map has been constructed in order to detect putative QTLs associated with this complex resistance.
The female map was comprised of 31 SDRF (Single Dose Restriction Fragment) markers assigned to 11 linkage groups giving a total size of 392.9 cM. On the male map, 52 SDRF markers mapped on 17 linkage groups giving a total size of 406.9 cM. The work is being continued with the addition of more markers on the maps.

Publication
Authors
E. Lerceteau-Köhler, P. Roudeillac, M. Markocic, G. Guérin, K. Praud, B. Denoyes-Rothan
Keywords
Colletotrichum acutatum, anthracnose, bulk segregant analysis, linkage maps, SDRF markers, AFLPs
Full text
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