Articles
INDUCTION OF ETHYLENE INSENSITIVITY INTO ONCIDIUM AND ODONTOGLOSSUM ORCHID SPECIES FOR IMPROVEMENT OF DISPLAY LIFE
Article number
906_32
Pages
253 – 257
Language
English
Abstract
Two orchid species, Oncidium and Odontoglossum, are sensitive to ethylene.
Exposure of cut inflorescences or potted flowering plants to 1 µl L-1 of ethylene accelerated bud drop, wilting of florets, and yellowing of leaves and pedicels.
To prevent ethylene effects two strategies have been used in our studies: 1) treatment with the ethylene receptor blocker 1-methylcyclopropene (1-MCP), 2) Agrobacterium mediated transformation with an etr1-1 mutant gene from Arabidopsis thaliana. Selected cultivars of Oncidium and Odontoglossum orchids were pre-treated with 200 nl L-1 1-MCP at 20°C for 6 h and subsequently exposed to 0 or 1 µl L-1 ethylene. 1-MCP clearly improved postharvest characteristics of both investigated species as well in presence as in the absence of ethylene.
Exposure of cut inflorescences or potted flowering plants to 1 µl L-1 of ethylene accelerated bud drop, wilting of florets, and yellowing of leaves and pedicels.
To prevent ethylene effects two strategies have been used in our studies: 1) treatment with the ethylene receptor blocker 1-methylcyclopropene (1-MCP), 2) Agrobacterium mediated transformation with an etr1-1 mutant gene from Arabidopsis thaliana. Selected cultivars of Oncidium and Odontoglossum orchids were pre-treated with 200 nl L-1 1-MCP at 20°C for 6 h and subsequently exposed to 0 or 1 µl L-1 ethylene. 1-MCP clearly improved postharvest characteristics of both investigated species as well in presence as in the absence of ethylene.
Authors
B. Raffeiner, M. Serek, T. Winkelmann
Keywords
1-methylcyclopropene, display life, flower senescence, postharvest quality
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