Articles
ISOLATION AND EXPRESSION OF ANTHOCYANIN PATHWAY RELATED GENES IN ACTINIDIA
Article number
913_12
Pages
111 – 116
Language
English
Abstract
Twelve genes related to anthocyanin synthesis were isolated from the red flesh of the kiwifruit cultivar, Actinidia chinensis var. chinensis Hongyang, by cDNA and SSH library sequencing and homologue cloning.
These genes include partial- or full-length structural genes, chalcone synthase (CHS), chalcone isomerase (CHI), flavanone 3-hydroxylase (F3H), dihydroflavonol 4-reductase (DFR), leucoanthocyanin deoxygenase (LDOX), UDP-glucose: flavonoid 3-O-glucosyltransferase (UFGT) and UDPG, regulatory genes as myeloblast (MYB) and tryptophanaspartate (WD) proteins, and transporting proteins such as multidrug resistanceassociated proteins(MRP)-like ABC transporters.
The isolated AcF3H ORF was 1101 bp in length, which encoded a 366 amino-acid peptide. AcF3H expression level was high before coloration, and then decreased at the early stage.
Real-time PCR showed that AcDFR1 expression was extremely high in a green-fleshed cultivar, A. chinensis var. deliciosa Jinkui, at anthesis, and remained very high during later development, but was lower in the yellow-fleshed cultivar, A. chinensis var. chinensis Jinnong, and the red-fleshed Hongyang. Expression of AcDFR2 was very low at all stages of Jinkui, but higher in Jinnong and Hongyang. Nevertheless, transcription levels of AcDFR1 and AcDFR2 increased with fruit coloration of the yellow/red-fleshed cultivars, especially in the later stages of development.
These genes include partial- or full-length structural genes, chalcone synthase (CHS), chalcone isomerase (CHI), flavanone 3-hydroxylase (F3H), dihydroflavonol 4-reductase (DFR), leucoanthocyanin deoxygenase (LDOX), UDP-glucose: flavonoid 3-O-glucosyltransferase (UFGT) and UDPG, regulatory genes as myeloblast (MYB) and tryptophanaspartate (WD) proteins, and transporting proteins such as multidrug resistanceassociated proteins(MRP)-like ABC transporters.
The isolated AcF3H ORF was 1101 bp in length, which encoded a 366 amino-acid peptide. AcF3H expression level was high before coloration, and then decreased at the early stage.
Real-time PCR showed that AcDFR1 expression was extremely high in a green-fleshed cultivar, A. chinensis var. deliciosa Jinkui, at anthesis, and remained very high during later development, but was lower in the yellow-fleshed cultivar, A. chinensis var. chinensis Jinnong, and the red-fleshed Hongyang. Expression of AcDFR2 was very low at all stages of Jinkui, but higher in Jinnong and Hongyang. Nevertheless, transcription levels of AcDFR1 and AcDFR2 increased with fruit coloration of the yellow/red-fleshed cultivars, especially in the later stages of development.
Publication
Authors
Y. Wang, H. Yang, J. Yang, Y. Man, L. Zhang, Z. Jiang, R. Qin, H. Huang
Keywords
Actinidia, anthocyanin, red-fleshed kiwifruit, genes, ‘Hongyang’
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