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Articles

CHARACTERIZATION OF PSEUDOMONAS SYRINGAE PV. ACTINIDIAE POPULATIONS USING DIFFERENT TYPING TECHNIQUES

Article number
913_66
Pages
495 – 499
Language
English
Abstract
Over the last few years a severe bacterial disease on kiwifruit has been reported in Italy; its causal agent was identified as Pseudomonas syringae pv. actinidiae. For epidemiological purposes, we evaluated the discriminatory power of various PCR-based fingerprinting approaches; profiles were generated by rep-PCR (including also (GTG)5-PCR), IS50-PCR, and RAPD, from a wide collection of Italian, Korean and Japanese strains, to investigate the genetic relationships between them and also with some representative strains of related pathovars.
It wasn’t possible to find dissimilarities among Italian strains, whilst some were evidenced between these and the Japanese/Korean strains of this pathovar.
Also, the other pathovars clustered separately, with the pathovar theae being always more closely related to actinidiae than the others.
In general, the results obtained with the different methods were in good agreement, with RAPD showing the highest values of divergence between groups.

Publication
Authors
A. Mazzaglia, M. Renzi, M.C. Taratufolo, G.M. Balestra
Keywords
kiwifruit, bacterial canker, genetic fingerprinting, RAPD, rep-PCR
Full text
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