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Articles

IN VITRO INDUCTION OF OCTOPLOID PLANTS FROM TETRAPLOID ACTINIDIA ARGUTA

Article number

913_23

Pages

185 – 190

Language

English

Abstract

Actinidia arguta produces greenish-yellow delicious fruits rich in vitamin C. To increase the fruit size of A. Arguta, octoploid plants were induced successfully treating in vitro leave pieces of tetraploid plants with colchicine.
Leaf pieces excised from in vitro shoots were treated with 15 different concentrations of colchicine in liquid MS medium supplemented with 6.66 μM BA and 0.64 μM IBA for 3, 5 or 7 days, respectively.
The results showed that the bud regeneration rate was affected by the concentration of colchicine and duration of treatment.
High concentrations and longer durations reduced the number of regenerated buds.
The optimum condition for inducing octoploids was to treat leaf pieces with 0.009% colchicine for 5 days.
Cytological analysis established that the chromosome number of tetraploid plants of A. arguta was 2n=4x=116 and that of octoploid plants was 2n=8x=232. Among the 111 regenerated plants from leave pieces treated with colchicine, 30 plants were confirmed as octoploid by chromosome counts and flow cytometry.

Publication

VII International Symposium on Kiwifruit

Authors

C. Liu, X. Sun, H. Dai, Z. Zhang

Keywords

chromosome doubling, colchicine, ploidy, tissue culture

Full text

https://doi.org/10.17660/ActaHortic.2011.913.23

Online Articles (96)

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22. ‘GOLDRUSH’, A NEWLY BRED KIWIFRUIT CULTIVAR IN KOREA

23. IN VITRO INDUCTION OF OCTOPLOID PLANTS FROM TETRAPLOID ACTINIDIA ARGUTA

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24. CHARACTERISTICS OF INTERSPECIFIC HYBRIDS BETWEEN ACTINIDIA CHINENSIS KIWIFRUIT AND A. RUFA NATIVE TO JAPAN

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R. Botondi | V. Russo | F. Mencarelli

78. INNOVATIVE NON-DESTRUCTIVE DEVICE FOR FRUIT QUALITY ASSESSMENT

G. Costa | E. Bonora | G. Fiori | M. Noferini

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T. Cooper | K. Sagredo | A. Mansilla | J. Streif

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D. Manríquez | B.G. Defilippi

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E. Sfakiotakis | I. Petkopoulou

83. SPATIAL DISTRIBUTION OF QUALITATIVE CHARACTERISTICS AND PRODUCTION FACTORS OF KIWIFRUIT AT “BEIRA LITORAL”, PORTUGAL

T. Panagopoulos | M.D.C. Antunes

84. TEN YEARS OF RESEARCH ON COMPLEMENTARY POLLINATION OF KIWIFRUIT

L. Asteggiano | L. Giordani | A. Bevilacqua | G. Vittone | G. Costa

85. POSTHARVEST APPLICATION OF 1-METHYLCYCLOPROPENE (1-MCP) EXTENDS SHELF LIFE OF KIWIFRUIT

C.M. Cantin | D. Holcroft | C.H. Crisosto

86. EVALUATION OF A NON-DESTRUCTIVE DRY MATTER SENSOR FOR KIWIFRUIT

C.M. Cantin | A. Soto | G.M. Crisosto | C.H. Crisosto

87. PRELIMINARY AGRONOMICAL AND NUTRACEUTICAL EVALUATION OF KIWIFRUIT GENOTYPES

88. 1-MCP IS AN EFFECTIVE TOOL FOR REDUCING DETRIMENTAL EFFECTS OF ETHYLENE CONTAMINATION DURING KIWIFRUIT STORAGE

B.G. Defilippi | P. Robledo | D. Manríquez

89. FATTY ACID AND MINERAL CONTENTS OF KIWIFRUIT SEED AND PULPS

E. Duman | M.M. Özcan

90. HIGHLY SENSITIVE ETHYLENE DETECTOR FOR ON-LINE MEASUREMENTS ON KIWIFRUITS

R. van den Dungen | S. Te Lintel Hekkert | S.M. Cristescu | F.J.M. Harren

91. TITRATABLE ACIDITY IN KIWIFRUIT, A COMPARISON OF DIFFERENT METHODS OF ANALYSIS

K.B. Marsh | M. Sullivan | T.G. Thorp

92. THE CHALLENGE OF GLOBALGAP^® STANDARD ON KIWIFRUIT

D. Ferrucci | N. Passeri | B. Pancino | D. Scipione

93. MONITORING OF ‘HAYWARD’ (ACTINIDIA DELICIOSA) FRUIT RIPENING IN NORTH-WEST ITALY

S. Pellegrino | F. Costamagna | M. Noferini | G. Costa

94. EFFECT OF HEAT SHRINKABLE FILMS ON SHELF LIFE, AND QUALITY OF TRAY-WRAPPED KIWIFRUITS (ACTINIDIA DELICIOSA CHEV.)

R.R. Sharma | R.K. Pal | S.K. Jha | A. Kumar

95. CHANGES IN VOLATILE PRODUCTION AND SENSORY QUALITY OF ACTINIDIA ARGUTA FRUIT DURING FRUIT MATURATION

M.Y. Wang | M. Wohlers | X. Chen | K.B. Marsh

96. FINAL DISCUSSION

A.R. Ferguson | G. Costa