Articles
BACTERIAL CANKER OF KIWIFRUIT (ACTINIDIA SPP.): PRELIMINARY BACTERIOLOGICAL STUDIES IN ASYMPTOMATIC PLANT MATERIALS IN NORTHEASTERN ITALY
Article number
913_68
Pages
509 – 516
Language
English
Abstract
Italy is one of the largest producers of Kiwi [Actinidia deliciosa (A. Chev.) C.F. Liang & A.R. Ferguson] and Hayward is the most widespread cultivar.
The development of reliable diagnostic methods for asymptomatic materials is crucial for the activity of Plant Protection Services in order to confirm the presence of the pathogen in the territory and in marketing materials.
To detect the presence of Pseudomanas syringae pv. actidiniae (Psa), bacteria isolation from the surface and the columellae of asymptomatic fruits harvested in Lazio region with epidemics of bacterial canker was performed.
For bacteria identification several biochemical tests and PCR analysis were carried out to discriminate Psa from the other kiwifruit pathogenic bacteria (i.e., Pseudomonas syringae pv. syringae and Pseudomonas viridiflava). The identification assays of the isolates obtained from the asymptomatic fruits of all samples excluded the presence of Psa.
However, Psa lives in the phyllosphere together with non-pathogenic bacteria that may interfere in the diagnosis of the bacterial canker by means of false negative or positive results.
Therefore, from leaves and shoots of asymptomatic plants of A. chinensis Hort16A and A. deliciosa Hayward, collected from the Emilia Romagna region, epiphytic/endophytic bacteria were isolated and studied as possible causes of interference in the diagnostic protocol.
The development of reliable diagnostic methods for asymptomatic materials is crucial for the activity of Plant Protection Services in order to confirm the presence of the pathogen in the territory and in marketing materials.
To detect the presence of Pseudomanas syringae pv. actidiniae (Psa), bacteria isolation from the surface and the columellae of asymptomatic fruits harvested in Lazio region with epidemics of bacterial canker was performed.
For bacteria identification several biochemical tests and PCR analysis were carried out to discriminate Psa from the other kiwifruit pathogenic bacteria (i.e., Pseudomonas syringae pv. syringae and Pseudomonas viridiflava). The identification assays of the isolates obtained from the asymptomatic fruits of all samples excluded the presence of Psa.
However, Psa lives in the phyllosphere together with non-pathogenic bacteria that may interfere in the diagnosis of the bacterial canker by means of false negative or positive results.
Therefore, from leaves and shoots of asymptomatic plants of A. chinensis Hort16A and A. deliciosa Hayward, collected from the Emilia Romagna region, epiphytic/endophytic bacteria were isolated and studied as possible causes of interference in the diagnostic protocol.
Publication
Authors
S. Mucini, E. Biondi, C. Lucchese, U. Mazzucchi, P. Minardi
Keywords
asymptomatic plant materials, bacterial canker, kiwifruit, PCR, phyllosphere, Pseudomonas syringae pv. actinidiae
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