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Articles

MORPHOGENESIS IN TISSUE CULTURES OF SOME MEDICINAL PLANTS

Article number
132_27
Pages
249 – 256
Language
Abstract
Regeneration capacities in vitro of some medicinal and spice plants (Matricaria chamomilla L., Achillea collina Becker, Centaurium erythraea Rafn. and Ligusticum mutellina (L.) Crantz.) have been investigated.
Morphogenesis and organogenesis from the meristematic parts of leaf explants and that from callus cultures have been tested under influence of various culture conditions.

Isolated leaves of Matricaria chamomilla and Achillea collina (Asteraceae) regenerated roots on Linsmaier and Skoog’s culture medium without phytohormones within ten days About 20 % of tested explants were able to regenerate shoots without any change of nutritional requirements

After supplementing the same culture medium with various concentrations of alpha -naphtylacetic acid the typical root system development without following shoot regeneration have been observed in concentrations of 0.5 ppm and 1.0 ppm.

Leaf explants and callus tissue of Centaurium erythraea (Gentianaceae) showed an expressive induction influence of benzyladenine on shoot regeneration.
Leaf sections isolated from plants of various age (4 to 12 weeks) as well as callus cultures were able to form leaves during cultivation on Linsmaier and Skoog’s culture medium supplemented with benzyladenine in concentrations of 0.1 to 3.0 ppm.
Regenerated plants started to develop roots after transferring them to the soil and during their adaptation to greenhouse conditions.

Callus cultures of Ligusticum mutellina (Daucaceae) cultivated on Murashige and Skoog’s culture medium with 0.5 ppm kinetin and 0 5 ppm alpha -naphtylacetic acid showed high morphogenetic potential.
Roots and shoots were regenerated simultaneously.

Publication
Authors
E. Cellárová, K. Repcáková, M. Repcák, R. Honcariv
Keywords
Full text
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