Articles
Effect of growth regulators and adenine sulphate on in vitro propagation of Agbagba (AAB) plantain
Article number
1348_44
Pages
319 – 325
Language
English
Abstract
The synergistic effect of adenine sulphate (AdS) and benzylaminopurine (BAP) on micropropagation of plantain (Agbagba) was investigated.
Apical meristems (2.0 cm) were excised from shoot tips of plantain and used as explants.
For regeneration, Murashige and Skoog (MS) medium was supplemented with different concentrations of BAP (2.0, 3.0, 4.0, 4.5 mg L‑1) plus 1.8 mg L‑1 IAA in combination with AdS (0, 50, 100 mg L‑1). Eight explants per treatment with three replicates were used.
It was observed that 83% of the shoots turned green 15 days after initiation on medium containing 4.0 mg L‑1 BAP + 1.8 mg L‑1 IAA + 100 mg L‑1 AdS. The highest shoot length (7.9 cm) and shoot weight (5.74 g) were also observed on the same medium 28 days after initiation.
Explants obtained from regeneration medium were passed through several cycles of subculture on MS medium supplemented with BAP (3.0, 4.0, 4.5, 5.0 mg L‑1) plus 1.8 mg L‑1 IAA in combination with AdS (50, 100, 150 mg L‑1). The highest mean number of shoots (23.33 explant‑1) and shoot length (5.87 cm explant‑1) were recorded in medium containing 4.5 mg L‑1 BAP + 1.8 mg L‑1 IAA + 100 mg L‑1 AdS at the third subculture.
This was followed by medium containing BAP 3.0 mg L‑1 + IAA 1.8 mg L‑1 + AdS 150 mg L‑1 which resulted in 19.67 shoots explant‑1 and shoots lengths of 6.03 cm explant‑1. A 3-fold increase was observed at the fourth subculture with MS medium supplemented with 4.5 mg L‑1 BAP + 1.8 mg L‑1 1AA + AdS 100 mg L‑1. Adenine sulphate in combination with BAP stimulates multiplication of Agbagba. An increase in AdS concentration did not result in a significant increase in multiplication rate.
Apical meristems (2.0 cm) were excised from shoot tips of plantain and used as explants.
For regeneration, Murashige and Skoog (MS) medium was supplemented with different concentrations of BAP (2.0, 3.0, 4.0, 4.5 mg L‑1) plus 1.8 mg L‑1 IAA in combination with AdS (0, 50, 100 mg L‑1). Eight explants per treatment with three replicates were used.
It was observed that 83% of the shoots turned green 15 days after initiation on medium containing 4.0 mg L‑1 BAP + 1.8 mg L‑1 IAA + 100 mg L‑1 AdS. The highest shoot length (7.9 cm) and shoot weight (5.74 g) were also observed on the same medium 28 days after initiation.
Explants obtained from regeneration medium were passed through several cycles of subculture on MS medium supplemented with BAP (3.0, 4.0, 4.5, 5.0 mg L‑1) plus 1.8 mg L‑1 IAA in combination with AdS (50, 100, 150 mg L‑1). The highest mean number of shoots (23.33 explant‑1) and shoot length (5.87 cm explant‑1) were recorded in medium containing 4.5 mg L‑1 BAP + 1.8 mg L‑1 IAA + 100 mg L‑1 AdS at the third subculture.
This was followed by medium containing BAP 3.0 mg L‑1 + IAA 1.8 mg L‑1 + AdS 150 mg L‑1 which resulted in 19.67 shoots explant‑1 and shoots lengths of 6.03 cm explant‑1. A 3-fold increase was observed at the fourth subculture with MS medium supplemented with 4.5 mg L‑1 BAP + 1.8 mg L‑1 1AA + AdS 100 mg L‑1. Adenine sulphate in combination with BAP stimulates multiplication of Agbagba. An increase in AdS concentration did not result in a significant increase in multiplication rate.
Publication
Authors
P.E. Akin-Idowu, A.O. Aduloju, Y.O. Olagunju, O.I. Akinyoola, G.U. Adebo
Keywords
Agbagba, adenine sulphate, benzylaminopurine, micropropagation, plantain
Groups involved
- Division Plant Genetic Resources, Breeding and Biotechnology
- Division Tropical and Subtropical Fruit and Nuts
- Division Temperate Tree Fruits
- Division Temperate Tree Nuts
- Division Vine and Berry Fruits
- Division Ornamental Plants
- Division Vegetables, Roots and Tubers
- Division Plant-Environment Interactions in Field Systems
- Division Greenhouse and Indoor Production Horticulture
- Division Postharvest and Quality Assurance
- Division Precision Horticulture and Engineering
- Division Horticulture for Human Health
- Division Sustaining Horticulture in a Changing World
- Division Landscape and Urban Horticulture
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