Articles
Factors affecting anther culture of ‘Galia’ melon (Cucumis melo L.)
Article number
1411_1
Pages
1 – 6
Language
English
Abstract
Anther culture is a method utilized for pure line production, and its success is influenced by the genotypes of the donor plants and cultural conditions.
The objectives of this study were to evaluate the effects of various factors on callus formation, including melon cultivars (‘Galia-green’ and ‘Galia-orange’), pretreatment temperatures (25 and 35°C), and induction media (M1: 0.50 mg L‑1 6-benzylaminopurine (BAP) and 1 mg L‑1 dichlorophenoxyacetic acid (2,4-D), and M2: 1 mg L‑1 BAP + 2 mg L‑1 2,4-D + 2 mg L‑1 kinetin (Kn). The experiment was conducted using a completely randomized design (CRD) in a factorial experiment with three factors and five replications.
After four weeks of being transferred to the induction media, it was observed that the interactions among the three factors studied had an effect on callus formation, which ranged from 57.64 to 90.05%. The interactions among the two factors studied did not have a significant impact on callus formation.
Regarding melon cultivars, ‘Galia-green’ (86.47%) exhibited a significantly higher percentage of callus formation compared to ‘Galia-orange’ (76.59%). Among the pretreatment temperatures, thermal shock at 25°C resulted in the highest callus formation with an average of 86.47%. Both induction media formulas induced callus formation, but the difference was not statistically significant.
The objectives of this study were to evaluate the effects of various factors on callus formation, including melon cultivars (‘Galia-green’ and ‘Galia-orange’), pretreatment temperatures (25 and 35°C), and induction media (M1: 0.50 mg L‑1 6-benzylaminopurine (BAP) and 1 mg L‑1 dichlorophenoxyacetic acid (2,4-D), and M2: 1 mg L‑1 BAP + 2 mg L‑1 2,4-D + 2 mg L‑1 kinetin (Kn). The experiment was conducted using a completely randomized design (CRD) in a factorial experiment with three factors and five replications.
After four weeks of being transferred to the induction media, it was observed that the interactions among the three factors studied had an effect on callus formation, which ranged from 57.64 to 90.05%. The interactions among the two factors studied did not have a significant impact on callus formation.
Regarding melon cultivars, ‘Galia-green’ (86.47%) exhibited a significantly higher percentage of callus formation compared to ‘Galia-orange’ (76.59%). Among the pretreatment temperatures, thermal shock at 25°C resulted in the highest callus formation with an average of 86.47%. Both induction media formulas induced callus formation, but the difference was not statistically significant.
Publication
Authors
N. Nitwatthanakul, A. Tira-umphon
Keywords
anther culture, pretreatment temperature, Galia melon
Groups involved
Online Articles (37)