Articles
BSA-seq mapping reveals QTLs for seed size and weight in bitter gourd (Momordica spp.)
Article number
1411_31
Pages
319 – 326
Language
English
Abstract
Bitter gourd belongs to the genus Momordica of the family Cucurbitaceae, it is originated in Africa and is now widely cultivated as a vegetable in Asia, eastern Africa, etc.
The seeds of bitter gourd have also been commonly used historically as folk medicine in China and India, and are rich in a variety of phytochemicals with pharmacological activity.
Bitter gourd seed size and weight vary widely across different germplasm resources, but the underlying genetic basis is largely unknown.
In this study, the large seeded bitter gourd inbred line K7-359 (M. charantia ssp. charantia) and the small seeded bitter gourd line K7-422 (M. charantia ssp. macroloba) were used as female and male parents, respectively, to produce F2 populations.
By using BSA-seq mapping approach, a total of 11, 12 and 10 QTLs were identified for seed length, seed width and single seed weight, respectively.
Of which, five genomic regions from chromosome 3, 6, 7 and 11 were found to be collectively associated with all of the three seed traits.
These findings will be useful for the downstream isolation of seed-related gene and other genetic applications in bitter gourd.
The seeds of bitter gourd have also been commonly used historically as folk medicine in China and India, and are rich in a variety of phytochemicals with pharmacological activity.
Bitter gourd seed size and weight vary widely across different germplasm resources, but the underlying genetic basis is largely unknown.
In this study, the large seeded bitter gourd inbred line K7-359 (M. charantia ssp. charantia) and the small seeded bitter gourd line K7-422 (M. charantia ssp. macroloba) were used as female and male parents, respectively, to produce F2 populations.
By using BSA-seq mapping approach, a total of 11, 12 and 10 QTLs were identified for seed length, seed width and single seed weight, respectively.
Of which, five genomic regions from chromosome 3, 6, 7 and 11 were found to be collectively associated with all of the three seed traits.
These findings will be useful for the downstream isolation of seed-related gene and other genetic applications in bitter gourd.
Publication
Authors
Jiaowen Cheng, Junjie Cui, Jia Liu, Yichao Zhou, Jianwen Song, Kailin Hu
Keywords
bitter gourd, seed size, seed weight, QTL, BSA-seq
Groups involved
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