Articles
Gene cloning and transcriptional analysis of a short fruit germplasm in cucumber
Article number
1411_3
Pages
15 – 34
Language
English
Abstract
Fruit length is one of the key components of appearance quality of cucumber, and it is also an important domestication trait that affects yield and commodity quality.
Despite some fruit length quantitative trait loci (QTLs) having been identified and several regulatory genes having been cloned, the gene network and underlying mechanism of fruit elongation remain largely unknown.
In this study, we identified a natural cucumber germplasm F305 with short fruit phenotype that is segregated from the inbred line F301. Sectioning analysis showed that the short fruit phenotype is due to reduced cell size in the longitudinal direction of F305. Bulked segregant analysis sequencing (BSA-seq) was performed using F301, F305 and their F2 population.
Our data identified a QTL FL1.1 located in the region 25093400-32481100 of chromosome 1, with a total length of about 7.39 Mb, was significantly associated with fruit length, in which contains the previously reported IQ-DOMAIN gene CsSUN. Short fruit F305 had a 161 bp deletion occurring in the first coding region of CsSUN gene resulted in premature termination of translation.
Therefore, CsSUN is the candidate gene of FL1.1. Cell sections showed that the CsSUN gene inhibits cell expansion in the longitudinal direction.
Transcriptomic analysis showed that CsSUN gene may regulate fruit elongation by participating in the biosynthesis and signal transduction of plant hormones such as auxin and ethylene.
In summary, we identified CsSUN gene accounts for the short fruit phenotype of a natural germplasm, providing a new material resource for further dissecting the mechanism of CsSUN regulating fruit growth and development in cucumber.
Despite some fruit length quantitative trait loci (QTLs) having been identified and several regulatory genes having been cloned, the gene network and underlying mechanism of fruit elongation remain largely unknown.
In this study, we identified a natural cucumber germplasm F305 with short fruit phenotype that is segregated from the inbred line F301. Sectioning analysis showed that the short fruit phenotype is due to reduced cell size in the longitudinal direction of F305. Bulked segregant analysis sequencing (BSA-seq) was performed using F301, F305 and their F2 population.
Our data identified a QTL FL1.1 located in the region 25093400-32481100 of chromosome 1, with a total length of about 7.39 Mb, was significantly associated with fruit length, in which contains the previously reported IQ-DOMAIN gene CsSUN. Short fruit F305 had a 161 bp deletion occurring in the first coding region of CsSUN gene resulted in premature termination of translation.
Therefore, CsSUN is the candidate gene of FL1.1. Cell sections showed that the CsSUN gene inhibits cell expansion in the longitudinal direction.
Transcriptomic analysis showed that CsSUN gene may regulate fruit elongation by participating in the biosynthesis and signal transduction of plant hormones such as auxin and ethylene.
In summary, we identified CsSUN gene accounts for the short fruit phenotype of a natural germplasm, providing a new material resource for further dissecting the mechanism of CsSUN regulating fruit growth and development in cucumber.
Publication
Authors
Yan Geng, Yuxiang Huang, Ye Liu, Chuang Li, Weiyuan Song, Liming Wang, Yafei Huang, Min Li, Jianyu Zhao, Xiaolan Zhang
Keywords
cucumber, fruit length, appearance quality, CsSUN, cell size
Groups involved
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