Articles
Comparative metabolomic and transcriptome analysis of seed vigor and aging in watermelon
Article number
1411_33
Pages
333 – 340
Language
English
Abstract
Seed deterioration during watermelon seed storage can lead to seed vigor loss, which adversely affects the mid- and long-term preservation of germplasm resources, and the conservation of species diversity.
However, the mechanism underlying seed vigor maintenance and seed aging remains largely unknown in watermelon.
In this study, a comparative metabolomic by liquid chromatography-mass spectrometry and transcriptome analysis was used to identify the differentially accumulated (DA) metabolites and differentially expressed genes (DEGs) related to seed vigor and aging in watermelon.
A total of 560 and 3304 common DA metabolites and DEGs were detected.
Our results suggested that plant hormone signal transduction and the phenylpropanoid biosynthesis pathway played an important role in regulating seed vigor and aging.
In addition, four DEGs were reported to play an indispensable role in seed vigor in other plants.
Of these DEGs, Cl1-CysPrxA related to 5-O-caffeoylshikimic acid and ClbZip23-1, ClbZip23-2 and ClbZip42 related jasmonic acid.
Notably, the content of jasmonic acid could be a potential candidate metabolomic biomarker for seed vigor and aging in watermelon.
Taken together, these findings are of great significance for the evaluation of seed vigor and the determination of seed quality for high vigor watermelon seeds.
However, the mechanism underlying seed vigor maintenance and seed aging remains largely unknown in watermelon.
In this study, a comparative metabolomic by liquid chromatography-mass spectrometry and transcriptome analysis was used to identify the differentially accumulated (DA) metabolites and differentially expressed genes (DEGs) related to seed vigor and aging in watermelon.
A total of 560 and 3304 common DA metabolites and DEGs were detected.
Our results suggested that plant hormone signal transduction and the phenylpropanoid biosynthesis pathway played an important role in regulating seed vigor and aging.
In addition, four DEGs were reported to play an indispensable role in seed vigor in other plants.
Of these DEGs, Cl1-CysPrxA related to 5-O-caffeoylshikimic acid and ClbZip23-1, ClbZip23-2 and ClbZip42 related jasmonic acid.
Notably, the content of jasmonic acid could be a potential candidate metabolomic biomarker for seed vigor and aging in watermelon.
Taken together, these findings are of great significance for the evaluation of seed vigor and the determination of seed quality for high vigor watermelon seeds.
Publication
Authors
Na Li, Shengnan Kong, Jianli Shang, Nannan Li, Dan Zhou, Jiming Wang, Shuangwu Ma
Keywords
watermelon, seed vigor, seed aging, metabolomic biomarkers
Groups involved
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