Articles
Silencing of the helix-loop-helix DNA-binding factor CsBEE1 compromises Fusarium wilt resistance in cucumber
Article number
1411_19
Pages
185 – 192
Language
English
Abstract
Cucumber (Cucumis sativus L.) Fusarium wilt (FW) is caused by the Fusarium oxysporum f. sp. cucumerinum (Foc) and generally accepted as a devastative soil-borne fungal disease, which severely impairs cucumber yield and quality worldwide.
Our previous studies showed that cucumber miR6300-CsBEE1 (helix-loop-helix DNA-binding factor, Csa1G024150) pair was significantly induced by FW via an integrated analysis of miRNA and transcriptome profiles, and quantitative real-time RT-PCR (qRT-PCR) assay.
However, the functions of CsBEE1 regulating cucumber FW resistance remain unknow.
Here, we used the virus-induced gene silencing (VIGS) method to silence the expression of CsBEE1 in cucumber, and found that the CsBEE1-silencing seedlings remarkedly decreased the resistance against cucumber FW. Furthermore, the expression of CsBEE1 was upregulated after salicylic acid (SA) treatment, and CsBEE1-silencing plants showed the inhibited expression of NPR1 in the SA signaling pathway, and the reduced SA content.
These findings indicate that CsBEE1 plays important roles in regulating cucumber defenses against FW, and provide the gene resources for developing new cucumber cultivars with improvement of wilt disease resistance.
Our previous studies showed that cucumber miR6300-CsBEE1 (helix-loop-helix DNA-binding factor, Csa1G024150) pair was significantly induced by FW via an integrated analysis of miRNA and transcriptome profiles, and quantitative real-time RT-PCR (qRT-PCR) assay.
However, the functions of CsBEE1 regulating cucumber FW resistance remain unknow.
Here, we used the virus-induced gene silencing (VIGS) method to silence the expression of CsBEE1 in cucumber, and found that the CsBEE1-silencing seedlings remarkedly decreased the resistance against cucumber FW. Furthermore, the expression of CsBEE1 was upregulated after salicylic acid (SA) treatment, and CsBEE1-silencing plants showed the inhibited expression of NPR1 in the SA signaling pathway, and the reduced SA content.
These findings indicate that CsBEE1 plays important roles in regulating cucumber defenses against FW, and provide the gene resources for developing new cucumber cultivars with improvement of wilt disease resistance.
Publication
Authors
Shuruo Mo, Rong Pan, Jiahao Shen, Ningyuan Zhang, Xuehao Chen, Jun Xu
Keywords
cucumber, Fusarium wilt, CsBEE1, VIGS, resistance
Groups involved
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