Articles
Optimised formulation of seed coating agent with Bacillus velezensis ZF145 for the control of Fusarium root rot in cucumber
Article number
1411_12
Pages
111 – 120
Language
English
Abstract
Seed coating with biocontrol bacteria is an effective and eco-friendly strategy to control soil-borne diseases.
However, microbial seed coating agents are often unsuccessfully formulated due to the poor compatibility of the adjuvants, which hinders their application.
In this study, a microbial seed coating agent (MSCA-ZF145) was successfully prepared, in which a biocontrol bacterium Bacillus velezensis ZF145 was used as active ingredient.
Recipe of the seed coating agent was optimised and the effect of different adjuvants on the survival rate of ZF145 was systematically investigated.
The cucumber seeds were evenly coated by shaking-plate method.
The physical-chemical performance of the MSCA-ZF145 was also investigated in terms of the coating uniformity, bacteria-loading content, and storage ability.
Importantly, Fusarium root rot disease was effectively controlled with a control efficacy of 57.9%, while the germination rate of cucumber seeds was not affected by MSCA-ZF145. Overall, this study provided a promising microbial seed coating method for the control of cucumber root rot.
However, microbial seed coating agents are often unsuccessfully formulated due to the poor compatibility of the adjuvants, which hinders their application.
In this study, a microbial seed coating agent (MSCA-ZF145) was successfully prepared, in which a biocontrol bacterium Bacillus velezensis ZF145 was used as active ingredient.
Recipe of the seed coating agent was optimised and the effect of different adjuvants on the survival rate of ZF145 was systematically investigated.
The cucumber seeds were evenly coated by shaking-plate method.
The physical-chemical performance of the MSCA-ZF145 was also investigated in terms of the coating uniformity, bacteria-loading content, and storage ability.
Importantly, Fusarium root rot disease was effectively controlled with a control efficacy of 57.9%, while the germination rate of cucumber seeds was not affected by MSCA-ZF145. Overall, this study provided a promising microbial seed coating method for the control of cucumber root rot.
Publication
Authors
Jialin Liu, Chengdong Hou, Lihong Liu, Yanxia Shi, Xuewen Xie, Ali Chai, Lei Li, Baoju Li, Youpu Cheng, Tengfei Fan
Keywords
biocontrol bacterium, microbial seed coating, soil-borne diseases, compatibility tests, adjuvants screening
Groups involved
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