Articles
Optimization of the cultivation and spore production system for gummy stem blight pathogen of melon
Article number
1411_13
Pages
121 – 128
Language
English
Abstract
In order to establish an efficient culture and spore production system for gummy stem blight (GSB) pathogen, the pathogen of Stagonosporopsis cucurbitacearum was isolated and purified from melon plant with typical GSB symptoms.
Then, the effects of different culture medium, incubation periods in the dark, light conditions, and duration of light exposure on spore production were systematically analyzed.
The results showed that the medium has a significant impact on spore production.
The highest spore yield of 3.69×107 spores mL‑1 was obtained on potato glucose tomato medium (PDT). Dark cultivation duration significantly affects the spore production.
At the temperature of 25°C, the spore production reached a peak of 2.35×107 spores mL‑1after 3 d of dark cultivation.
Different lighting conditions and duration also significantly affect spore production.
Cultivation under continuous ultraviolet lighting had the highest spore production of 3.65×107 spores mL‑1. Additionally, ultraviolet lighting duration also had a significant effect on spore production.
When exposed to ultraviolet lamps continuously for 5 d, the spore production reached up to 7.86×107 spores mL‑1. Taken together, the optimal culture condition for rapid and efficient production of melon GSB pathogenic spores is using the PDT medium and cultivation under darkness at 25°C for 3 d, and then continuous ultraviolet lighting for 5 d.
Then, the effects of different culture medium, incubation periods in the dark, light conditions, and duration of light exposure on spore production were systematically analyzed.
The results showed that the medium has a significant impact on spore production.
The highest spore yield of 3.69×107 spores mL‑1 was obtained on potato glucose tomato medium (PDT). Dark cultivation duration significantly affects the spore production.
At the temperature of 25°C, the spore production reached a peak of 2.35×107 spores mL‑1after 3 d of dark cultivation.
Different lighting conditions and duration also significantly affect spore production.
Cultivation under continuous ultraviolet lighting had the highest spore production of 3.65×107 spores mL‑1. Additionally, ultraviolet lighting duration also had a significant effect on spore production.
When exposed to ultraviolet lamps continuously for 5 d, the spore production reached up to 7.86×107 spores mL‑1. Taken together, the optimal culture condition for rapid and efficient production of melon GSB pathogenic spores is using the PDT medium and cultivation under darkness at 25°C for 3 d, and then continuous ultraviolet lighting for 5 d.
Publication
Authors
Yaqiong Li, Xiaoying Wei, Tinglin Wen, Zhengzhu Wang, Jirong Bian, Haiyan Wang, Qiusheng Kong
Keywords
melon, gummy stem blight, Stagonosporopsis cucurbitacearum, sporulation system, optimization
Groups involved
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