Articles
Genomic sequencing and functional gene research of luffa
Article number
1411_6
Pages
55 – 66
Language
English
Abstract
Luffa is an important cultivated vegetable and medicinal plant in the family Cucurbitaceae. There are nine different species of the Luffa genus, of which only Luffa acutangula (L.) Roxb. and L. cylindrica (L.) Roem. are domesticated.
In this study, the draft genome sequences of the L. cylindrica inbred line P93075 was analyzed.
Using Illumina, PacBio, and 10× Genomics sequencing techniques as well as new assembly techniques such as FALCON and chromatin interaction mapping (Hi-C), a chromosome-scale genome of approximately 656.19 Mb with a N50 scaffold of 48.76 Mb was generated.
From this assembly, 25,508 protein-coding gene loci were identified, with 63.81% of the whole genome consisting of transposable elements, which are major contributors to the expansion of the luffa genome.
Based on obtaining genomic data of luffa, we constructed various genetic populations to finely map and clone important agronomic trait genes such as dwarf phenotype, fruit length and powdery mildew resistance.
These results will greatly promote the development of molecular markers assisted breeding for luffa.
In this study, the draft genome sequences of the L. cylindrica inbred line P93075 was analyzed.
Using Illumina, PacBio, and 10× Genomics sequencing techniques as well as new assembly techniques such as FALCON and chromatin interaction mapping (Hi-C), a chromosome-scale genome of approximately 656.19 Mb with a N50 scaffold of 48.76 Mb was generated.
From this assembly, 25,508 protein-coding gene loci were identified, with 63.81% of the whole genome consisting of transposable elements, which are major contributors to the expansion of the luffa genome.
Based on obtaining genomic data of luffa, we constructed various genetic populations to finely map and clone important agronomic trait genes such as dwarf phenotype, fruit length and powdery mildew resistance.
These results will greatly promote the development of molecular markers assisted breeding for luffa.
Publication
Authors
Haibin Wu, Gangjun Zhao, Jianning Luo, Lili Liu, Junxing Li, Hao Gong, Xiaoming Zheng, Xiaoxi Liu, Liting Deng, Junqiu Chen
Keywords
Luffa, genome sequences, gene
Groups involved
Online Articles (37)