Articles
Inheritance analysis and genetic mapping of the full green peel color in Cucurbita maxima
Article number
1411_7
Pages
67 – 78
Language
English
Abstract
Fruit peel color is an important quality trait.
The full-green peel pumpkin is a popular variety preferred by consumers.
The inheritance of the full green peel (Gf) is largely unknown.
To identify its inheritance, we selected red peel ‘Diaogua’ (P1) and green peel ‘Agol’ (P2) pumpkin inbred lines to develop their progenies.
All the peel colors of F1 showed mixed bicolors of red and green fruit, which indicates no complete dominance of red peel over green peel.
Depending on the percentage of red parts on fruits of F2, the peel colors were classified into five categories: grade 1 (red), grade 2 (red-and-green bicolor; red background with green stripes), grade 3 (red-and-green; red parts are approximately equal to green parts), grade 4 (green-and-red bicolor; green background with red stripes), and grade 5 (green). Based on the above categories, of the 117 BC1P1 individuals, 95, 14 and 8 individuals revealed fruit grades 1, 2 and 3, respectively, while there were no grades 4 and 5. From the 109 BC1P2, fruit grades 3, 4, and 5 were included in 10, 57, and 42 individuals, respectively, while there were no grades 1 and 2. In the BC1P2 population, there should have been 55 individuals expected to produce green peel in theory, while only 42 individuals revealed green peel color in practice, which has some deviation.
From 210 F2, 173 individuals showed non-green peel, and only 37 individuals showed green peel, which suggested that the deviation is increased in the F2 population.
A major gene controls the red peel, but there may still be several minor genes affecting the red peel formation.
Furthermore, SSR marker-based mapping reveals that the full green (Gf) gene is located on linkage group 7, and the nearest marker is PU000529, with a genetic distance of 6.30 cM. This study may lay a genetic foundation for full green peel gene mapping and cloning.
The full-green peel pumpkin is a popular variety preferred by consumers.
The inheritance of the full green peel (Gf) is largely unknown.
To identify its inheritance, we selected red peel ‘Diaogua’ (P1) and green peel ‘Agol’ (P2) pumpkin inbred lines to develop their progenies.
All the peel colors of F1 showed mixed bicolors of red and green fruit, which indicates no complete dominance of red peel over green peel.
Depending on the percentage of red parts on fruits of F2, the peel colors were classified into five categories: grade 1 (red), grade 2 (red-and-green bicolor; red background with green stripes), grade 3 (red-and-green; red parts are approximately equal to green parts), grade 4 (green-and-red bicolor; green background with red stripes), and grade 5 (green). Based on the above categories, of the 117 BC1P1 individuals, 95, 14 and 8 individuals revealed fruit grades 1, 2 and 3, respectively, while there were no grades 4 and 5. From the 109 BC1P2, fruit grades 3, 4, and 5 were included in 10, 57, and 42 individuals, respectively, while there were no grades 1 and 2. In the BC1P2 population, there should have been 55 individuals expected to produce green peel in theory, while only 42 individuals revealed green peel color in practice, which has some deviation.
From 210 F2, 173 individuals showed non-green peel, and only 37 individuals showed green peel, which suggested that the deviation is increased in the F2 population.
A major gene controls the red peel, but there may still be several minor genes affecting the red peel formation.
Furthermore, SSR marker-based mapping reveals that the full green (Gf) gene is located on linkage group 7, and the nearest marker is PU000529, with a genetic distance of 6.30 cM. This study may lay a genetic foundation for full green peel gene mapping and cloning.
Publication
Authors
K. Gebretsadik, Chenggang Xiang, Ying Duan, Kailiang Bo, Changlin Wang
Keywords
Cucurbita maxima, red, green, incomplete dominance, fruit color, SSR
Groups involved
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