Articles
COAT PROTEIN-MEDIATED PROTECTION AGAINST PLUM POX VIRUS
Article number
336_10
Pages
85 – 92
Language
Abstract
In the last few years, successful examples of resistance breeding against viruses from several different plant virus families have been reported, using the coat protein-mediated cross protection approach.
In order to verify the validity of this approach against Plum Pox Virus (PPV), leaf explants from herbaceous host plants, Nicotiana clevelandii and Nicotiana benthamiana were transformed with Agrobacterium tumefaciens strain LBA 4404 containing the plasmid pBinPPVm.
Following inoculation, plants were investigated for development of symptoms and virus accumulation.
The phenomenon of virus resistance was observed at different levels when transgenic plants, expressing the coat protein (CP + ), and control plants (CP-) were compared after challenge infection with PPV. Nicotiana clevelandii CP + plants circumvented virus accumulation.
After an initial increase in virus titer similar to the control plants, some CP + plants showed a reduced accumulation of virus and inhibition of the systematic spread, characterized by decrease of the virus titer and formation of new symptomless leaves.
In other Nicotiana clevelandii CP + plants, virus accumulation was inhibited and disease symptoms never appeared. Nicotiana benthamiana CP + plants were also protected.
After a temporary virus accumulation, virus titer decreased without the appearance of symptoms with the exception of a few plants, which showed a delay of 30 days in the development of symptoms post challenge infection.
In order to verify the validity of this approach against Plum Pox Virus (PPV), leaf explants from herbaceous host plants, Nicotiana clevelandii and Nicotiana benthamiana were transformed with Agrobacterium tumefaciens strain LBA 4404 containing the plasmid pBinPPVm.
Following inoculation, plants were investigated for development of symptoms and virus accumulation.
The phenomenon of virus resistance was observed at different levels when transgenic plants, expressing the coat protein (CP + ), and control plants (CP-) were compared after challenge infection with PPV. Nicotiana clevelandii CP + plants circumvented virus accumulation.
After an initial increase in virus titer similar to the control plants, some CP + plants showed a reduced accumulation of virus and inhibition of the systematic spread, characterized by decrease of the virus titer and formation of new symptomless leaves.
In other Nicotiana clevelandii CP + plants, virus accumulation was inhibited and disease symptoms never appeared. Nicotiana benthamiana CP + plants were also protected.
After a temporary virus accumulation, virus titer decreased without the appearance of symptoms with the exception of a few plants, which showed a delay of 30 days in the development of symptoms post challenge infection.
A system was also developed that allows the transfer of foreign genes into apricot (Prunus armeniaca) cultivars.
Transgenic apricot plants were obtained following co-cultivation with Agrobacterium tumefaciens strain LBA 4404 containing various binary plasmids, pBinGUSint, carrying the marker gene 
-glucuronidase (GUS) and pBinPPVm, carrying the coat protein gene of Plum Pox Virus (PPV).
Authors
M. Machado, A. Machado, V. Hanzer, H. Weiss, F. Regner, H. Steinkellner, R. Plail, E. Knapp, H. Katinger
Keywords
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