Articles
FACTORS INFLUENCING THE OCCURRENCE OF SOMACLONAL VARIATIONS IN MICROPROPAGATED BANANAS
Article number
336_47
Pages
357 – 364
Language
Abstract
Initial explants were cultured on 16 different media and, during a period of 420 days, plantlets were subcultured every 6 weeks.
Plantlets developed were divided into two classes: (a) single, fully developed large plantlets (L) that formed a bulbous-like corm, and (b) plantlets whose growth and corm formation was suppressed and resulted in formation of a few to many basal small plantlets (A) without distinct basal corm (suspected to be adventitious). The bulb of the L plantlets was split longitudinally when they reached a size of three leaves.
Each half was subcultured for further multiplication.
During each subculture, A plantlets were separated from the L plantlets and transferred to the field.
Some of the L plantlets were also transferred to the field.
The following details were kept for each of 4136 field evaluated plants: initial explant and generation number, kind of medium and plantlets (L or A). The main conclusions were: (a) the rate of variants obtained was not affected by medium composition or by the rate of multiplication; (b) the rate of variants was lowest in L plants and much higher in A plants; (c) the length of time in culture was not found to be a mutation-inducing factor, since some variants were found among plants derived from the initial explants; (d) the initial explant was found to be the main factor that determined the occurrence of somaclonal variants in forming ‘stable’ and ‘non-stable’ families obtained from these explants.
Plantlets developed were divided into two classes: (a) single, fully developed large plantlets (L) that formed a bulbous-like corm, and (b) plantlets whose growth and corm formation was suppressed and resulted in formation of a few to many basal small plantlets (A) without distinct basal corm (suspected to be adventitious). The bulb of the L plantlets was split longitudinally when they reached a size of three leaves.
Each half was subcultured for further multiplication.
During each subculture, A plantlets were separated from the L plantlets and transferred to the field.
Some of the L plantlets were also transferred to the field.
The following details were kept for each of 4136 field evaluated plants: initial explant and generation number, kind of medium and plantlets (L or A). The main conclusions were: (a) the rate of variants obtained was not affected by medium composition or by the rate of multiplication; (b) the rate of variants was lowest in L plants and much higher in A plants; (c) the length of time in culture was not found to be a mutation-inducing factor, since some variants were found among plants derived from the initial explants; (d) the initial explant was found to be the main factor that determined the occurrence of somaclonal variants in forming ‘stable’ and ‘non-stable’ families obtained from these explants.
Authors
O. Reuveni, S. Golubowicz, Y. Israeli
Keywords
Musa acuminata L., plant growth regulators, stable families
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